Organization of the mouse RNA-specific adenosine deaminase Adar1 gene 5′-region and demonstration of STAT1-independent, STAT2-dependent transcriptional activation by interferon
Copyright policy )Organization of the mouse RNA-specific adenosine deaminase Adar1 gene 5′-region and demonstration of STAT1-independent, STAT2-dependent transcriptional activation by interferon
The p150 form of the RNA-specific adenosine deaminase ADAR1 is interferon-inducible and catalyzes A-to-I editing of viral and cellular RNAs. We have characterized mouse genomic clones containing the promoter regions required for Adar1 gene transcription and analyzed interferon induction of the p150 protein using mutant mouse cell lines. Transient transfection analyses using reporter constructs led to the identification of three promoters, one interferon-inducible (P(A)) and two constitutively active (P(B) and P(C)). The TATA-less P(A) promoter, characterized by the presence of a consensus ISRE element and a PKR kinase KCS-like element, directed interferon-inducible reporter expression in rodent and human cells. Interferon induction of p150 was impaired in mouse cells deficient in IFNAR receptor, JAK1 kinase or STAT2 but not STAT1. Whereas Adar1 gene organization involving multiple promoters and alternative exon 1 structures was highly preserved, sequences of the promoters and exon 1 structures were not well conserved between human and mouse.
- University of California, San Francisco United States
- University of California System United States
- IFN Germany
- University of California, Santa Barbara United States
Signal Transducers and Activators of Transcription (STAT), Adenosine Deaminase, Molecular Sequence Data, Receptor, Interferon alpha-beta, Cell Line, Mice, Genes, Reporter, Virology, Animals, Humans, Luciferases, Promoter Regions, Genetic, Conserved Sequence, Binding Sites, Base Sequence, RNA-Binding Proteins, STAT2 Transcription Factor, Janus Kinase 1, Sequence Analysis, DNA, Artificial Gene Fusion, STAT1 Transcription Factor, Adenosine Deaminase Acting on RNA (ADAR), Interferon, Interferons
Signal Transducers and Activators of Transcription (STAT), Adenosine Deaminase, Molecular Sequence Data, Receptor, Interferon alpha-beta, Cell Line, Mice, Genes, Reporter, Virology, Animals, Humans, Luciferases, Promoter Regions, Genetic, Conserved Sequence, Binding Sites, Base Sequence, RNA-Binding Proteins, STAT2 Transcription Factor, Janus Kinase 1, Sequence Analysis, DNA, Artificial Gene Fusion, STAT1 Transcription Factor, Adenosine Deaminase Acting on RNA (ADAR), Interferon, Interferons
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