Thiamine repression and pyruvate decarboxylase autoregulation independently control the expression of the Saccharomyces cerevisiae PDC5 gene
Copyright policy )Thiamine repression and pyruvate decarboxylase autoregulation independently control the expression of the Saccharomyces cerevisiae PDC5 gene
The Saccharomyces cerevisiae gene PDC5 encodes the minor isoform of pyruvate decarboxylase (Pdc). In this work we show that expression of PDC5 but not that of PDC1, which encodes the major isoform, is repressed by thiamine. Hence, under thiamine limitation both PDC1 and PDC5 are expressed. PDC5 also becomes strongly expressed in a pdc1Δ mutant. Two‐dimensional gel electrophoresis of whole protein extracts shows that thiamine limitation stimulates the production of THI gene products and of Pdc5p. Deletion of PDC1 only stimulates production of Pdc5p. We conclude that the stimulation of PDC5 expression in a pdc1Δ mutant is not due to a response to thiamine limitation.
- KU Leuven Belgium
- Katholieke Universiteit Leuven Belgium
- KATHOLIEKE UNIVERSITEIT LEUVEN Belgium
- Université Catholique de Louvain Belgium
- University of Gothenburg Sweden
Recombinant Fusion Proteins, Autoregulation, Saccharomyces cerevisiae, Yeast, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Fungal, Electrophoresis, Gel, Two-Dimensional, Gene expression, Thiamine, Pyruvate decarboxylase, Promoter Regions, Genetic, Pyruvate Decarboxylase
Recombinant Fusion Proteins, Autoregulation, Saccharomyces cerevisiae, Yeast, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Fungal, Electrophoresis, Gel, Two-Dimensional, Gene expression, Thiamine, Pyruvate decarboxylase, Promoter Regions, Genetic, Pyruvate Decarboxylase
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