ABSTRACT Are the microtubule-organising centers of the different cell types of a metazoan interchangeable? If not, what are the differences between them? Do they play any role in the differentiation processes to which these cells are subjected? Nearly one hundred years of centrosome research has established the essential role of this organelle as the main microtubule-organising center of animal cells. But only now are we starting to unveil the answers to the challenging questions which are raised when the centrosome is studied within the context of a pluricellular organism. In this review we present some of the many examples which illustrate how centrosomes and microtubule organisation changes through development in Drosophila and discuss some of its implications.