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Biological Procedures Online
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A rapid and easy method for the DNA extraction from Cryptococcus neoformans

descriptionPublicationkeyboard_double_arrow_right Article , Other literature type 21 Jul 2011 English Publisher:Springer Science and Business Media LLCJournal:Biological Procedures Online, volume 13 (eissn: 1480-9222, Copyright policy )
Authors: Sellami Hayet; Sellami Amira; Jarboui Mohammed; Mseddi Fatma; Ayadi Ali;

doi: 10.1186/1480-9222-13-5

pmid: 21777412

pmc: PMC3156736

A rapid and easy method for the DNA extraction from Cryptococcus neoformans

Abstract

Abstract DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension. This procedure is simpler than previously reported methods.

Keywords

Medicine (General), R5-920, QH301-705.5, Biochemistry, Genetics and Molecular Biology(all), Research, Biology (General)

  • BIP!
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    citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 11
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    		 Average
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    		 Average
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    		 Average
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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
11
Average
Average
Average
Green
gold
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