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Development
Article . 2001 . Peer-reviewed
Data sources: Crossref
Development
Article . 2001
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Sonic hedgehog is a survival factor for hypaxial muscles during mouse development

Authors: S. Mundlos; R. Schafer; Detlev Mennerich; Thomas Braun; Marcus Krüger; S. Fees;

Sonic hedgehog is a survival factor for hypaxial muscles during mouse development

Abstract

ABSTRACT Sonic hedgehog (Shh) has been proposed to function as an inductive and trophic signal that controls development of epaxial musculature in vertebrate embryos. In contrast, development of hypaxial muscles was assumed to occur independently of Shh. We here show that formation of limb muscles was severely affected in two different mouse strains with inactivating mutations of the Shh gene. The limb muscle defect became apparent relatively late and initial stages of hypaxial muscle development were unaffected or only slightly delayed. Micromass cultures and cultures of tissue fragments derived from limbs under different conditions with or without the overlaying ectoderm indicated that Shh is required for the maintenance of the expression of myogenic regulatory factors (MRFs) and, consecutively, for the formation of differentiated limb muscle myotubes. We propose that Shh acts as a survival and proliferation factor for myogenic precursor cells during hypaxial muscle development. Detection of a reduced but significant level of Myf5 expression in the epaxial compartment of somites of Shh homozygous mutant embryos at E9.5 indicated that Shh might be dispensable for the initiation of myogenesis both in hypaxial and epaxial muscles. Our data suggest that Shh acts similarly in both somitic compartments as a survival and proliferation factor and not as a primary inducer of myogenesis.

Keywords

Limb Buds, Dishevelled Proteins, Muscle Proteins, Cell Differentiation, Extremities, Immunohistochemistry, Mice, Mutant Strains, DNA-Binding Proteins, Mesoderm, Embryonic and Fetal Development, Mice, Cell Movement, Bone Morphogenetic Proteins, Ectoderm, Animals, Hedgehog Proteins, Muscle, Skeletal, Cells, Cultured, In Situ Hybridization, Adaptor Proteins, Signal Transducing

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Powered by OpenAIRE graph
citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
110
Top 10%
Top 10%
Top 1%