Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2
Copyright policy )Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2
Indole-3-acetaldoxime (IAOx) is a key branching point between primary and secondary metabolism. IAOx serves as an intermediate in the biosynthesis of indole glucosinolates (I-GLSs), camalexin and the plant hormone indole-3-acetic acid (IAA). The cytochrome P450s CYP79B2 and CYP79B3 catalyze the conversion of tryptophan to IAOx. CYP83B1 channels IAOx into I-GLS biosynthesis, CYP71A13 channels IAOx into camalexin biosynthesis, whereas the IAOx-metabolizing enzyme in IAA biosynthesis is not known. In this report, we demonstrate controlled production of I-GLSs by introducing an ethanol (EtOH)-inducible CYP79B2 construct into double (cyp79b2 cyp79b3) or triple (cyp79b2 cyp79b3 cyp83b1) mutant lines. We show EtOH-dependent induction of camalexin and identify a number of candidate IAA homeostasis- or defense-related genes by clustered microarray analysis. The transgenic mutant lines are thus promising tools for elucidating the interplay between primary and secondary metabolism.
- University of Copenhagen Denmark
- Technical University of Denmark Denmark
- University of Copenhagen Denmark
Indoles, Ethanol, Indoleacetic Acids, Molecular Structure, Arabidopsis Proteins, Gene Expression Profiling, Glucosinolates, Arabidopsis, Plants, Genetically Modified, Gene Expression Regulation, Enzymologic, Mass Spectrometry, Biosynthetic Pathways, Thiazoles, Cytochrome P-450 Enzyme System, Gene Expression Regulation, Plant, Mutation, Oximes, Cluster Analysis, Chromatography, Liquid, Oligonucleotide Array Sequence Analysis
Indoles, Ethanol, Indoleacetic Acids, Molecular Structure, Arabidopsis Proteins, Gene Expression Profiling, Glucosinolates, Arabidopsis, Plants, Genetically Modified, Gene Expression Regulation, Enzymologic, Mass Spectrometry, Biosynthetic Pathways, Thiazoles, Cytochrome P-450 Enzyme System, Gene Expression Regulation, Plant, Mutation, Oximes, Cluster Analysis, Chromatography, Liquid, Oligonucleotide Array Sequence Analysis
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).14 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!