GABA influences the development of the ventromedial nucleus of the hypothalamus
Copyright policy )GABA influences the development of the ventromedial nucleus of the hypothalamus
AbstractThe region that becomes the ventromedial nucleus of the hypothalamus (VMH) is surrounded by cells and fibers containing immunoreactive gamma‐aminobutyric acid (GABA) by embryonic day 13 (E13), several days before the nucleus emerges in Nissl stains. As GABA plays many roles during neural development, we hypothesized that it influences VMH development, perhaps by providing boundary information for migrating neurons. To test this hypothesis we examined the VMH in embryonic mice in which the β3 subunit of the GABAA‐receptor, a receptor subunit that is normally highly expressed in this nucleus, was disrupted by gene targeting. In β3 −/− embryos the VMH was significantly larger, and the distribution of cells containing immunoreactive estrogen receptor‐α was expanded compared to controls. Using in vitro brain slices from wild‐type C57BL/6J mice killed at E15 we found that treatment with the GABAA antagonist bicuculline increased the number of cells migrating per video field analyzed in the VMH. In addition, treatment with either bicuculline or the GABAA agonist muscimol altered the orientation of cell migration in particular regions of this nucleus. These data suggest that GABA is important for the organization of cells during VMH formation. © 2001 John Wiley & Sons, Inc. J Neurobiol 49: 264–276, 2001
- University of Pittsburgh United States
- University of Vienna Austria
Brain Chemistry, Microscopy, Video, Estrogen Receptor alpha, Gene Expression Regulation, Developmental, In Vitro Techniques, Receptors, GABA-A, Immunohistochemistry, GABA Antagonists, Mice, Inbred C57BL, Mice, Receptors, Estrogen, Pregnancy, Ventromedial Hypothalamic Nucleus, Animals, Female, GABA-A Receptor Agonists, GABA-A Receptor Antagonists, GABA Agonists, gamma-Aminobutyric Acid, Fluorescent Dyes
Brain Chemistry, Microscopy, Video, Estrogen Receptor alpha, Gene Expression Regulation, Developmental, In Vitro Techniques, Receptors, GABA-A, Immunohistochemistry, GABA Antagonists, Mice, Inbred C57BL, Mice, Receptors, Estrogen, Pregnancy, Ventromedial Hypothalamic Nucleus, Animals, Female, GABA-A Receptor Agonists, GABA-A Receptor Antagonists, GABA Agonists, gamma-Aminobutyric Acid, Fluorescent Dyes
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).43 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!