<b><i>Background:</i></b> Aquaglyceroporin-7 <i>(AQP7)</i> is an adipose glycerol channel protein that has been suggested to be involved in whole-body glucose homeostasis and insulin sensitivity. The aim of this study was to investigate the expression of <i>AQP7</i> in visceral adipose tissues from women with the polycystic ovary syndrome (PCOS). <b><i>Methods:</i></b><i>AQP7</i> mRNA and protein levels were measured in omental adipose tissue from 5 women with the PCOS and 4 healthy controls matched for body mass index and age; this was done by the real-time polymerase chain reaction (PCR) and Western blotting. -<b><i>Results:</i></b> The women with the PCOS had significantly higher homeostasis model insulin resistance indices (HOMA_IR) and their quantitative insulin sensitivity check indices were significantly lower compared to the controls (p < 0.05). No significant difference was noted between the two groups for the plasma glycerol concentration. <i>AQP7</i> mRNA expression and protein levels in omental adipose tissue from women with the PCOS were significantly higher than those of the controls (p = 0.007). <i>AQP7</i> expression showed a positive correlation with fasting insulin levels, insulin levels at 2 h after glucose loading and HOMA_IR in women with the PCOS. <b><i>Conclusion:</i></b><i>AQP7</i> overexpression may be related to insulin sensitivity and glucose homeostasis in women with the PCOS.