Localization of the FAR3 gene: Genetic mapping and molecular cloning using a chromosome walk‐‘n’‐roll strategy
Copyright policy )Localization of the FAR3 gene: Genetic mapping and molecular cloning using a chromosome walk‐‘n’‐roll strategy
AbstractFAR3 is a newly‐discovered yeast gene required specifically for pheromone‐mediated cell cycle arrest. I have used strains harboring the far3‐1 mutation to map the gene to the right arm of chromosome XIII, establishing the gene order CEN13‐LYS7‐MCM1‐FAR3. I cloned the FAR3 gene based on its genetic map position using a strategy that combined chromosome walking and a related technique termed ‘chromosome rolling’. In addition to the genetic and physical localization of FAR3, I present data that suggest corrections to the tentative map positions of VAN1 and ARG80.
- University of Oregon United States
Saccharomyces cerevisiae Proteins, Cell Cycle, Genes, Fungal, Restriction Mapping, Chromosome Mapping, Cell Cycle Proteins, Saccharomyces cerevisiae, Fungal Proteins, Chromosome Walking, Chromosomes, Fungal, Cloning, Molecular, Crosses, Genetic
Saccharomyces cerevisiae Proteins, Cell Cycle, Genes, Fungal, Restriction Mapping, Chromosome Mapping, Cell Cycle Proteins, Saccharomyces cerevisiae, Fungal Proteins, Chromosome Walking, Chromosomes, Fungal, Cloning, Molecular, Crosses, Genetic
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