Effects of Ser2 and Tyr6 Mutants of BAF53 on Cell Growth and p53-dependent Transcription
Copyright policy )Effects of Ser2 and Tyr6 Mutants of BAF53 on Cell Growth and p53-dependent Transcription
BAF53 is an actin-related protein that shuttles between nucleus and cytoplasm. In the nucleus, it constitutes an integral component of many chromatin-modifying complexes such as the SWI/SNF, TIP60, TRRAP, and TIP48/49 complexes. BAF53 is essential for growth, but its function remains elusive. BAF53 homologues from yeast to humans have a conserved N-terminal motif, MS_(G/A)(G/A)_(V/L)YGG, which is unique to these proteins. Previously we showed that over-expression of an N-terminal deletion mutant of BAF53 (BAF53_deltaN) reduced the viability of HEK293 and HeLa cells. When we replaced the serine 2 and tyrosine 6 of this N-terminal motif with alanine, over-expression of the alanine-replaced BAF53 strongly impaired the growth of HEK293 cells whereas replacement with aspartate/glutamate had no effect. The alanine-replaced BAF53 mutants also stimulated p53-dependent transcription, in which the SWI/SNF and TRRAP complexes are involved. Our results demonstrate that serine 2 and tyrosine 6 play important roles in BAF53 activity.
- Dankook University Korea (Republic of)
- Hankuk University of Foreign Studies Korea (Republic of)
Alanine, Transcription, Genetic, Chromosomal Proteins, Non-Histone, Nuclear Proteins, Kidney, Actins, Chromatin, DNA-Binding Proteins, Mutation, Serine, Humans, Tyrosine, Tumor Suppressor Protein p53, Promoter Regions, Genetic, Cells, Cultured, Adaptor Proteins, Signal Transducing, Cell Proliferation, Transcription Factors
Alanine, Transcription, Genetic, Chromosomal Proteins, Non-Histone, Nuclear Proteins, Kidney, Actins, Chromatin, DNA-Binding Proteins, Mutation, Serine, Humans, Tyrosine, Tumor Suppressor Protein p53, Promoter Regions, Genetic, Cells, Cultured, Adaptor Proteins, Signal Transducing, Cell Proliferation, Transcription Factors
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