The Survival of Motor Neuron (SMN) Protein Interacts with the mRNA-Binding Protein HuD and Regulates Localization of Poly(A) mRNA in Primary Motor Neuron Axons
The Survival of Motor Neuron (SMN) Protein Interacts with the mRNA-Binding Protein HuD and Regulates Localization of Poly(A) mRNA in Primary Motor Neuron Axons
Spinal muscular atrophy (SMA) results from reduced levels of the survival of motor neuron (SMN) protein, which has a well characterized function in spliceosomal small nuclear ribonucleoprotein assembly. Currently, it is not understood how deficiency of a housekeeping protein leads to the selective degeneration of spinal cord motor neurons. Numerous studies have shown that SMN is present in neuronal processes and has many interaction partners, including mRNA-binding proteins, suggesting a potential noncanonical role in axonal mRNA metabolism. In this study, we have established a novel technological approach using bimolecular fluorescence complementation (BiFC) and quantitative image analysis to characterize SMN-protein interactions in primary motor neurons. Consistent with biochemical studies on the SMN complex, BiFC analysis revealed that SMN dimerizes and interacts with Gemin2 in nuclear gems and axonal granules. In addition, using pull down assays, immunofluorescence, cell transfection, and BiFC, we characterized a novel interaction between SMN and the neuronal mRNA-binding protein HuD, which was dependent on the Tudor domain of SMN. A missense mutation in the SMN Tudor domain, which is known to cause SMA, impaired the interaction with HuD, but did not affect SMN axonal localization or self-association. Furthermore, time-lapse microscopy revealed SMN cotransport with HuD in live motor neurons. Importantly, SMN knockdown in primary motor neurons resulted in a specific reduction of both HuD protein and poly(A) mRNA levels in the axonal compartment. These findings reveal a noncanonical role for SMN whereby its interaction with mRNA-binding proteins may facilitate the localization of associated poly(A) mRNAs into axons.
- University of Milan Italy
- Istituto Auxologico Italiano Italy
- Emory University United States
- Yeshiva University United States
- Istituti di Ricovero e Cura a Carattere Scientifico Italy
Motor Neurons, Blotting, Western, Fluorescent Antibody Technique, SMN Complex Proteins, Chick Embryo, ELAV-Like Protein 4, Hippocampus, Poly(A)-Binding Proteins, Axons, Rats, Mice, HEK293 Cells, Prosencephalon, ELAV Proteins, Animals, Humans, Immunoprecipitation, Poly A, In Situ Hybridization, Fluorescence
Motor Neurons, Blotting, Western, Fluorescent Antibody Technique, SMN Complex Proteins, Chick Embryo, ELAV-Like Protein 4, Hippocampus, Poly(A)-Binding Proteins, Axons, Rats, Mice, HEK293 Cells, Prosencephalon, ELAV Proteins, Animals, Humans, Immunoprecipitation, Poly A, In Situ Hybridization, Fluorescence
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