Cyclin E2: a novel CDK2 partner in the late G1 and S phases of the mammalian cell cycle
Copyright policy )Cyclin E2: a novel CDK2 partner in the late G1 and S phases of the mammalian cell cycle
We report here the cloning and characterization of human and mouse cyclin E2, which define a new subfamily within the vertebrate E-type cyclins, while all previously identified family-members belong to the cyclin El subfamily. Cyclin E2/CKD2 and cyclin E/CDK2 complexes phosphorylate histone H1 in vitro with similar specific activities and both are inhibited by p27Kip1. Cyclin E2 mRNA levels in human cells oscillate throughout the cell cycle and peak at the G1/S boundary, in parallel with the cyclin E mRNA. In cells, cyclin E2 is complexed with CDK2, p27 and p21. Like cyclin E, cyclin E2 is an unstable protein in vivo and is stabilized by proteasome inhibitors. Cyclin E2-associated kinase activity rises in late G1 and peaks very close to cyclin E activity. In two malignantly transformed cell lines, cyclin E2 activity is sustained throughout S phase, while cyclin E activity has already declined and cyclin A activity is only beginning to rise. We speculate that cyclin E2 is not simply redundant with cyclin E, but may regulate distinct rate-limiting pathway(s) in G1-S control.
- University of Toronto Canada
- ISREC Switzerland
- University of Geneva Switzerland
Cyclin-Dependent Kinase Inhibitor p21, Drosophila melanogaster/genetics, S Phase/ physiology, Molecular Sequence Data, Histones/metabolism, Cell Cycle Proteins, 616.07, Histones, Cyclin-Dependent Kinases/ physiology, Mice, G1 Phase/ physiology, Species Specificity, Cyclins, CDC2-CDC28 Kinases, Animals, Drosophila Proteins, Humans, Amino Acid Sequence, Phosphorylation, Cloning, Molecular, Protein-Serine-Threonine Kinases/ physiology, Sequence Homology, Amino Acid, Tumor Suppressor Proteins, Cell Cycle, Cyclin-Dependent Kinase 2, Nucleopolyhedrovirus/genetics, G1 Phase, Cyclin-Dependent Kinases, Nucleopolyhedroviruses, Drosophila melanogaster, Microtubule-Associated Proteins/metabolism, Cell Cycle/physiology, Cyclins/genetics/metabolism/ physiology, Protein Processing, Post-Translational, Sequence Alignment, Microtubule-Associated Proteins, Cyclin-Dependent Kinase Inhibitor p27, ddc: ddc:616.07
Cyclin-Dependent Kinase Inhibitor p21, Drosophila melanogaster/genetics, S Phase/ physiology, Molecular Sequence Data, Histones/metabolism, Cell Cycle Proteins, 616.07, Histones, Cyclin-Dependent Kinases/ physiology, Mice, G1 Phase/ physiology, Species Specificity, Cyclins, CDC2-CDC28 Kinases, Animals, Drosophila Proteins, Humans, Amino Acid Sequence, Phosphorylation, Cloning, Molecular, Protein-Serine-Threonine Kinases/ physiology, Sequence Homology, Amino Acid, Tumor Suppressor Proteins, Cell Cycle, Cyclin-Dependent Kinase 2, Nucleopolyhedrovirus/genetics, G1 Phase, Cyclin-Dependent Kinases, Nucleopolyhedroviruses, Drosophila melanogaster, Microtubule-Associated Proteins/metabolism, Cell Cycle/physiology, Cyclins/genetics/metabolism/ physiology, Protein Processing, Post-Translational, Sequence Alignment, Microtubule-Associated Proteins, Cyclin-Dependent Kinase Inhibitor p27, ddc: ddc:616.07
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).138 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!