Coupling of Cell Energetics with Membrane Metabolic Sensing
Copyright policy )Coupling of Cell Energetics with Membrane Metabolic Sensing
Transduction of metabolic signals is essential in preserving cellular homeostasis. Yet, principles governing integration and synchronization of membrane metabolic sensors with cell metabolism remain elusive. Here, analysis of cellular nucleotide fluxes and nucleotide-dependent gating of the ATP-sensitive K+ (K(ATP)) channel, a prototypic metabolic sensor, revealed a diffusional barrier within the submembrane space, preventing direct reception of cytosolic signals. Creatine kinase phosphotransfer, captured by 18O-assisted 31P NMR, coordinated tightly with ATP turnover, reflecting the cellular energetic status. The dynamics of high energy phosphoryl transfer through the creatine kinase relay permitted a high fidelity transmission of energetic signals into the submembrane compartment synchronizing K(ATP) channel activity with cell metabolism. Knock-out of the creatine kinase M-CK gene disrupted signal delivery to K(ATP) channels and generated a cellular phenotype with increased electrical vulnerability. Thus, in the compartmentalized cell environment, phosphotransfer systems shunt diffusional barriers and secure regimented signal transduction integrating metabolic sensors with the cellular energetic network.
- Mayo Clinic United States
- Radboud University Nijmegen Netherlands
Mice, Knockout, Magnetic Resonance Spectroscopy, Potassium Channels, Myocardium, Cell Membrane, Creatine Kinase, MM Form, Heart, Study of abnormal differentiation and transformation processes in heritable and acquired disorders with the use of cell and animal models, Models, Biological, Membrane Potentials, Adenosine Diphosphate, Isoenzymes, Kinetics, Mice, Adenosine Triphosphate, Bestudering van abnormale differentiatie en transformatieprocessen bij erfelijke of verworven aandoeningen m.b.v. cel- en diermodellen, Animals, Energy Metabolism, Creatine Kinase
Mice, Knockout, Magnetic Resonance Spectroscopy, Potassium Channels, Myocardium, Cell Membrane, Creatine Kinase, MM Form, Heart, Study of abnormal differentiation and transformation processes in heritable and acquired disorders with the use of cell and animal models, Models, Biological, Membrane Potentials, Adenosine Diphosphate, Isoenzymes, Kinetics, Mice, Adenosine Triphosphate, Bestudering van abnormale differentiatie en transformatieprocessen bij erfelijke of verworven aandoeningen m.b.v. cel- en diermodellen, Animals, Energy Metabolism, Creatine Kinase
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