Ret rescues mitochondrial morphology and muscle degeneration of Drosophila Pink1 mutants
Copyright policy )Ret rescues mitochondrial morphology and muscle degeneration of Drosophila Pink1 mutants
Parkinson's disease (PD)-associated Pink1 and Parkin proteins are believed to function in a common pathway controlling mitochondrial clearance and trafficking. Glial cell line-derived neurotrophic factor (GDNF) and its signaling receptor Ret are neuroprotective in toxin-based animal models of PD. However, the mechanism by which GDNF/Ret protects cells from degenerating remains unclear. We investigated whether the Drosophila homolog of Ret can rescue Pink1 and park mutant phenotypes. We report that a signaling active version of Ret (Ret(MEN₂B) rescues muscle degeneration, disintegration of mitochondria and ATP content of Pink1 mutants. Interestingly, corresponding phenotypes of park mutants were not rescued, suggesting that the phenotypes of Pink1 and park mutants have partially different origins. In human neuroblastoma cells, GDNF treatment rescues morphological defects of PINK1 knockdown, without inducing mitophagy or Parkin recruitment. GDNF also rescues bioenergetic deficits of PINK knockdown cells. Furthermore, overexpression of Ret(MEN₂B) significantly improves electron transport chain complex I function in Pink1 mutant Drosophila. These results provide a novel mechanism underlying Ret-mediated cell protection in a situation relevant for human PD.
- Max Planck Institute of Neurobiology Germany
- Max Planck Society Germany
- Ruhr University Bochum Germany
- Helmholtz Association of German Research Centres Germany
- Ludwig-Maximilians-Universität München Germany
pharmacology [Glial Cell Line-Derived Neurotrophic Factor], Dopamine, prevention & control [Muscular Atrophy], Apoptosis, park protein, Drosophila, PINK1 protein, Drosophila, Neuroblastoma, Adenosine Triphosphate, genetics [Drosophila Proteins], physiology [Electron Transport Complex I], pathology [Neuroblastoma], Drosophila Proteins, metabolism [Dopamine], ultrastructure [Neurons], genetics [Ubiquitin-Protein Ligases], Neurons, genetics [Drosophila melanogaster], genetics [Protein Kinases], Pupa, deficiency [Protein Kinases], genetics [Proto-Oncogene Proteins c-ret], Parkinson Disease, Articles, Protein-Serine-Threonine Kinases, ultrastructure [Mitochondria, Muscle], Muscular Atrophy, Drosophila melanogaster, Phenotype, PTEN-induced putative kinase, Ret protein, Drosophila, Ubiquitin-Protein Ligases, deficiency [Ubiquitin-Protein Ligases], genetics [Protein-Serine-Threonine Kinases], Oxygen Consumption, Cell Line, Tumor, physiology [Signal Transduction], Autophagy, Animals, Humans, Glial Cell Line-Derived Neurotrophic Factor, physiology [Protein-Serine-Threonine Kinases], Electron Transport Complex I, Proto-Oncogene Proteins c-ret, deficiency [Drosophila Proteins], physiology [Drosophila Proteins], Mitochondria, Muscle, deficiency [Protein-Serine-Threonine Kinases], Disease Models, Animal, physiology [Proto-Oncogene Proteins c-ret], metabolism [Adenosine Triphosphate], Genes, Lethal, growth & development [Drosophila melanogaster], Protein Kinases, ddc: ddc:570
pharmacology [Glial Cell Line-Derived Neurotrophic Factor], Dopamine, prevention & control [Muscular Atrophy], Apoptosis, park protein, Drosophila, PINK1 protein, Drosophila, Neuroblastoma, Adenosine Triphosphate, genetics [Drosophila Proteins], physiology [Electron Transport Complex I], pathology [Neuroblastoma], Drosophila Proteins, metabolism [Dopamine], ultrastructure [Neurons], genetics [Ubiquitin-Protein Ligases], Neurons, genetics [Drosophila melanogaster], genetics [Protein Kinases], Pupa, deficiency [Protein Kinases], genetics [Proto-Oncogene Proteins c-ret], Parkinson Disease, Articles, Protein-Serine-Threonine Kinases, ultrastructure [Mitochondria, Muscle], Muscular Atrophy, Drosophila melanogaster, Phenotype, PTEN-induced putative kinase, Ret protein, Drosophila, Ubiquitin-Protein Ligases, deficiency [Ubiquitin-Protein Ligases], genetics [Protein-Serine-Threonine Kinases], Oxygen Consumption, Cell Line, Tumor, physiology [Signal Transduction], Autophagy, Animals, Humans, Glial Cell Line-Derived Neurotrophic Factor, physiology [Protein-Serine-Threonine Kinases], Electron Transport Complex I, Proto-Oncogene Proteins c-ret, deficiency [Drosophila Proteins], physiology [Drosophila Proteins], Mitochondria, Muscle, deficiency [Protein-Serine-Threonine Kinases], Disease Models, Animal, physiology [Proto-Oncogene Proteins c-ret], metabolism [Adenosine Triphosphate], Genes, Lethal, growth & development [Drosophila melanogaster], Protein Kinases, ddc: ddc:570
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