Death-associated Protein 3 Regulates Mitochondrial-encoded Protein Synthesis and Mitochondrial Dynamics
Copyright policy )Death-associated Protein 3 Regulates Mitochondrial-encoded Protein Synthesis and Mitochondrial Dynamics
Mitochondrial morphologies change over time and are tightly regulated by dynamic machinery proteins such as dynamin-related protein 1 (Drp1), mitofusion 1/2, and optic atrophy 1 (OPA1). However, the detailed mechanisms of how these molecules cooperate to mediate fission and fusion remain elusive. DAP3 is a mitochondrial ribosomal protein that involves in apoptosis, but its biological function has not been well characterized. Here, we demonstrate that DAP3 specifically localizes in the mitochondrial matrix. Knockdown of DAP3 in mitochondria leads to defects in mitochondrial-encoded protein synthesis and abnormal mitochondrial dynamics. Moreover, depletion of DAP3 dramatically decreases the phosphorylation of Drp1 at Ser-637 on mitochondria, enhancing the retention time of Drp1 puncta on mitochondria during the fission process. Furthermore, autophagy is inhibited in the DAP3-depleted cells, which sensitizes cells to different types of death stimuli. Together, our results suggest that DAP3 plays important roles in mitochondrial function and dynamics, providing new insights into the mechanism of a mitochondrial ribosomal protein function in cell death.
- National University of Singapore Singapore
Dynamins, Ribosomal Proteins, Membrane Proteins, RNA-Binding Proteins, Mitochondrial Dynamics, Cell Line, Mitochondria, Mitochondrial Proteins, Gene Knockout Techniques, Mice, Protein Transport, Gene Knockdown Techniques, Protein Biosynthesis, Autophagy, Animals, Homeostasis, Humans, Phosphorylation, RNA, Small Interfering, Apoptosis Regulatory Proteins
Dynamins, Ribosomal Proteins, Membrane Proteins, RNA-Binding Proteins, Mitochondrial Dynamics, Cell Line, Mitochondria, Mitochondrial Proteins, Gene Knockout Techniques, Mice, Protein Transport, Gene Knockdown Techniques, Protein Biosynthesis, Autophagy, Animals, Homeostasis, Humans, Phosphorylation, RNA, Small Interfering, Apoptosis Regulatory Proteins
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).33 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!