Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains
Copyright policy )Nucleosome mobilization by ISW2 requires the concerted action of the ATPase and SLIDE domains
The ISWI family of ATP-dependent chromatin remodelers represses transcription by changing nucleosome positions. ISWI regulates nucleosome positioning by requiring a minimal length of extranucleosomal DNA for moving nucleosomes. ISW2 from Saccharomyces cerevisiae, a member of the ISWI family, has a conserved domain called SLIDE (SANT-like ISWI domain) that binds to extranucleosomal DNA ~19 base pairs from the edge of nucleosomes. Loss of SLIDE binding does not perturb binding of the ATPase domain or the initial movement of DNA inside of nucleosomes. Not only is extranucleosomal DNA required to help recruit ISW2, but also the interactions of the SLIDE domain with extranucleosomal DNA are functionally required to move nucleosomes.
- Department of Chemistry and Chemical Biology Harvard University United States
- Howard Hughes Medical Institute University of Washington United States
- Howard Hughes Medical Institute United States
- Southern Illinois University Carbondale United States
- University of California, San Francisco United States
570, Protein Structure, Biomedical and clinical sciences, Blotting, Western, Biophysics, 610, Centrifugation, Photoaffinity Labels, Saccharomyces cerevisiae, Medical and Health Sciences, Article, Gene Expression Regulation, Fungal, Genetics, Centrifugation, Density Gradient, Fluorescence Resonance Energy Transfer, DNA, Fungal, Adenosine Triphosphatases, Blotting, Hydroxyl Radical, DNA, Biological Sciences, Chromatin Assembly and Disassembly, Nucleosomes, Protein Structure, Tertiary, Biological sciences, Density Gradient, Fungal, Gene Expression Regulation, Chemical sciences, Chemical Sciences, Biochemistry and Cell Biology, Western, Tertiary, Developmental Biology, Transcription Factors
570, Protein Structure, Biomedical and clinical sciences, Blotting, Western, Biophysics, 610, Centrifugation, Photoaffinity Labels, Saccharomyces cerevisiae, Medical and Health Sciences, Article, Gene Expression Regulation, Fungal, Genetics, Centrifugation, Density Gradient, Fluorescence Resonance Energy Transfer, DNA, Fungal, Adenosine Triphosphatases, Blotting, Hydroxyl Radical, DNA, Biological Sciences, Chromatin Assembly and Disassembly, Nucleosomes, Protein Structure, Tertiary, Biological sciences, Density Gradient, Fungal, Gene Expression Regulation, Chemical sciences, Chemical Sciences, Biochemistry and Cell Biology, Western, Tertiary, Developmental Biology, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).57 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!