The Human Renal Sodium Sulfate Cotransporter (SLC13A1; hNaSi-1) cDNA and Gene: Organization, Chromosomal Localization, and Functional Characterization
Copyright policy )The Human Renal Sodium Sulfate Cotransporter (SLC13A1; hNaSi-1) cDNA and Gene: Organization, Chromosomal Localization, and Functional Characterization
Sulfate plays an essential role during growth, development, bone/cartilage formation, and cellular metabolism. In this study, we have determined the structure of the human Na+-sulfate cotransporter (hNaSi-1) cDNA (Human Genome Nomenclature Committee-approved symbol SLC13A1) and gene (NAS1). hNaSi-1 encodes a protein of 595 amino acids with 13 putative transmembrane domains. hNaSi-1 mRNA expression was exclusive to the human kidney. Expression of hNaSi-1 protein in Xenopus oocytes demonstrated a high-affinity Na+-sulfate cotransporter that was inhibited by selenate, thiosulfate, molybdate, tungstate, citrate, and succinate. Antisense inhibition experiments suggest hNaSi-1 to represent the major Na+-sulfate cotransporter in the human kidney. NAS1 was localized on human chromosome 7, mapped to 7q31-q32, near the sulfate transporter genes, DRA and SUT-1. The NAS1 gene contains 15 exons, spanning over 83 kb in length. Knowledge of the structure, function, and chromosomal localization of hNaSi-1 will permit the screening of NAS1 mutations in humans with disorders in renal sulfate reabsorption and homeostasis.
- University of Queensland Australia
- University of Queensland Australia
- University of Queensland Australia
DNA, Complementary, Xenopus, Molecular Sequence Data, Kidney, C1, 616, Genetics, Animals, Humans, Amino Acid Sequence, Cloning, Molecular, Cation Transport Proteins, DNA Primers, Sodium Sulfate Cotransporter, Base Sequence, Sequence Homology, Amino Acid, Symporters, 270000 Biological Sciences, 730000 - Health, Chromosome Mapping, Exons, Introns, Carrier Proteins, Chromosomes, Human, Pair 7
DNA, Complementary, Xenopus, Molecular Sequence Data, Kidney, C1, 616, Genetics, Animals, Humans, Amino Acid Sequence, Cloning, Molecular, Cation Transport Proteins, DNA Primers, Sodium Sulfate Cotransporter, Base Sequence, Sequence Homology, Amino Acid, Symporters, 270000 Biological Sciences, 730000 - Health, Chromosome Mapping, Exons, Introns, Carrier Proteins, Chromosomes, Human, Pair 7
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