Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56
Copyright policy )Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56
The human 56-kD U2AF65-associated protein (hUAP56), a member of the DExD/H box protein family of RNA-dependent ATPases, is required for the stable binding of U2 snRNP to the pre-mRNA branchpoint. Here we identify a highly conserved Saccharomyces cerevisiae homolog of hUAP56, yUAP/Sub2p. yUAP/Sub2p can be functionally substituted for by hUAP56 and, like its human counterpart, is an essential pre-mRNA splicing factor. yUAP/Sub2p is required for formation of the commitment complex, the precursor for U2 snRNP addition. In conjunction with previous studies, we conclude that at least two DExD/H box proteins, Prp5p and yUAP/Sub2p, mediate the U2 snRNP-branchpoint interaction.
- University of Massachusetts Medical School United States
- Howard Hughes Medical Institute United States
Adenosine Triphosphatases, 570, Sequence Homology, Amino Acid, RNA Splicing, Molecular Sequence Data, Nuclear Proteins, Saccharomyces cerevisiae, Splicing Factor U2AF, Recombinant Proteins, Fungal Proteins, Ribonucleoproteins, Mutagenesis, Site-Directed, Humans, Amino Acid Sequence, Cloning, Molecular, Sequence Alignment, Conserved Sequence
Adenosine Triphosphatases, 570, Sequence Homology, Amino Acid, RNA Splicing, Molecular Sequence Data, Nuclear Proteins, Saccharomyces cerevisiae, Splicing Factor U2AF, Recombinant Proteins, Fungal Proteins, Ribonucleoproteins, Mutagenesis, Site-Directed, Humans, Amino Acid Sequence, Cloning, Molecular, Sequence Alignment, Conserved Sequence
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