RAB-27 and its effector RBF-1 regulate the tethering and docking steps of DCV exocytosis in C. elegans
Copyright policy )RAB-27 and its effector RBF-1 regulate the tethering and docking steps of DCV exocytosis in C. elegans
The molecular mechanisms by which dense core vesicles (DCVs) translocate, tether, dock and prime are poorly understood. In this study, Caenorhabditis elegans was used as a model organism to study the function of Rab proteins and their effectors in DCV exocytosis. RAB-27/AEX-6, but not RAB-3, was found to be required for peptide release from neurons. By analyzing the movement of DCVs approaching the plasma membrane using total internal reflection fluorescence microscopy, we demonstrated that RAB-27/AEX-6 is involved in the tethering of DCVs and that its effector rabphilin/RBF-1 is required for the initial tethering and subsequent stabilization by docking.
- Institute of Biophysics China (People's Republic of)
- Huazhong University of Science and Technology China (People's Republic of)
- Chinese Academy of Sciences (中国科学院) China (People's Republic of)
- Chinese Academy of Sciences China (People's Republic of)
- Chinese Academy of Science (中国科学院) China (People's Republic of)
Green Fluorescent Proteins, Vesicular Transport Proteins, Nerve Tissue Proteins, Rabphilin-3A, Models, Biological, Exocytosis, rab27 GTP-Binding Proteins, Environmental Science(all), Spectroscopy, Fourier Transform Infrared, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Embryonic Stem Cells, Adaptor Proteins, Signal Transducing, Neurons, Agricultural and Biological Sciences(all), Biochemistry, Genetics and Molecular Biology(all), Secretory Vesicles, Cell Membrane, Biological Transport, rab GTP-Binding Proteins, Peptides, Protein Binding
Green Fluorescent Proteins, Vesicular Transport Proteins, Nerve Tissue Proteins, Rabphilin-3A, Models, Biological, Exocytosis, rab27 GTP-Binding Proteins, Environmental Science(all), Spectroscopy, Fourier Transform Infrared, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Embryonic Stem Cells, Adaptor Proteins, Signal Transducing, Neurons, Agricultural and Biological Sciences(all), Biochemistry, Genetics and Molecular Biology(all), Secretory Vesicles, Cell Membrane, Biological Transport, rab GTP-Binding Proteins, Peptides, Protein Binding
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