Molecular cloning of the CD45-associated 30-kDa protein.
Copyright policy )Molecular cloning of the CD45-associated 30-kDa protein.
CD45, a leukocyte-specific transmembrane protein tyrosine phosphatase, mediates signal transduction pathways critical for immune responses. However, the mechanism of CD45-mediated signal transduction and the identity of CD45-associated proteins have remained unclear. A CD45-associated 30-kDA phosphorylated protein (CD45-AP) was purified by virtue of its specific association with CD45, and its mouse cDNA was cloned by using the internal amino acid sequence information. In vitro translated CD45-AP bound specifically to CD45. CD45-AP appears to be leukocyte-specific and shares no significant homology with presently known sequences. The predicted sequence contains no consensus tyrosine phosphorylation sites or conserved sequences of GTP-binding proteins. CD45-AP may act as an adapter molecule for CD45-mediated signal transduction.
- Roger Williams Medical Center United States
- Brown University United States
Male, Mice, Inbred BALB C, DNA, Complementary, Base Sequence, Molecular Sequence Data, Intracellular Signaling Peptides and Proteins, Membrane Proteins, Chromatography, Affinity, Cell Line, Molecular Weight, Blotting, Southern, Kinetics, Mice, Organ Specificity, Leukocytes, Animals, Leukocyte Common Antigens, Amino Acid Sequence, Cloning, Molecular, Gene Library
Male, Mice, Inbred BALB C, DNA, Complementary, Base Sequence, Molecular Sequence Data, Intracellular Signaling Peptides and Proteins, Membrane Proteins, Chromatography, Affinity, Cell Line, Molecular Weight, Blotting, Southern, Kinetics, Mice, Organ Specificity, Leukocytes, Animals, Leukocyte Common Antigens, Amino Acid Sequence, Cloning, Molecular, Gene Library
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