Regulation of microtubule minus-end dynamics by CAMSAPs and Patronin
Copyright policy )Regulation of microtubule minus-end dynamics by CAMSAPs and Patronin
Significance Many microtubule (MT) plus-end regulators have been described, but regulation of MT minus-ends remains poorly understood. Recently, the related calmodulin-regulated spectrin-associated proteins (CAMSAPs) CAMSAP2/3 (vertebrate) and Patronin ( Drosophila ) were shown to bind minus-ends, but their effects on MT dynamics in vitro have not been studied. Here, by using in vitro assays, we show that minus-end binding and stabilization are properties of all CAMSAPs. However, their effects on minus-end growth and decoration are variable among different members of this family. Together, these studies solidify the role of CAMSAPs/Patronin as MT minus-end regulatory proteins and reveal how evolution has tuned their activities to affect minus-ends in distinct ways.
- University of California, San Francisco United States
570, Protein Structure, 1.1 Normal biological development and functioning, Kinesins, Microtubules, Polymerization, Tubulin, Animals, Drosophila Proteins, Humans, cytoskeletal regulation, Kinesin, Biological Sciences, Protein Structure, Tertiary, Health Disparities, tubulin polymerization, Cytoskeletal Proteins, Protein Subunits, Drosophila melanogaster, Biochemistry and Cell Biology, Microtubule-Associated Proteins, Tertiary, TIRF microscopy, Protein Binding
570, Protein Structure, 1.1 Normal biological development and functioning, Kinesins, Microtubules, Polymerization, Tubulin, Animals, Drosophila Proteins, Humans, cytoskeletal regulation, Kinesin, Biological Sciences, Protein Structure, Tertiary, Health Disparities, tubulin polymerization, Cytoskeletal Proteins, Protein Subunits, Drosophila melanogaster, Biochemistry and Cell Biology, Microtubule-Associated Proteins, Tertiary, TIRF microscopy, Protein Binding
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