De-Novo Cloning of FKBP23 cDNA from Pig ER Using Nested PCR
Copyright policy )De-Novo Cloning of FKBP23 cDNA from Pig ER Using Nested PCR
FK506 binding proteins (FKBPs) in cells are known as immunophilins. We have identifi ed and characterized a cDNA encoding an endoplasmic reticulum (ER) immunophilin, FKBP23, from pig liver by nested PCR. The predicted amino acid sequence of pig FKBP23 shows high identity to those of human FKBP23 and mouse FKBP23. It possesses a conserved FKBP-type peptidylprolyl cis-trans isomerase (PPIase) domain and EF-hand domain. We constructed a plasmid to express pFKBP23. Furthermore, we proved that the recombinant pFKBP23 can specifi cally bind to natural BiP, the main protein of the molecular chaperone Hsp70 in ER lumen; the binding is interrelated with the Ca2+ concentration just as the FKBP23 from mice.
- Nankai University China (People's Republic of)
Base Sequence, Swine, Calcium-Binding Proteins, Molecular Sequence Data, Endoplasmic Reticulum, Polymerase Chain Reaction, Tacrolimus Binding Proteins, Mice, Animals, Humans, Amino Acid Sequence, Cloning, Molecular
Base Sequence, Swine, Calcium-Binding Proteins, Molecular Sequence Data, Endoplasmic Reticulum, Polymerase Chain Reaction, Tacrolimus Binding Proteins, Mice, Animals, Humans, Amino Acid Sequence, Cloning, Molecular
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