This paper presents a survey of spider species that have not been previously recorded for Israel. Twenty species, twelve genera and two families (Mysmenidae and Phyxelididae) are recorded for the first time in Israel. Nine species, Agroeca parva Bosmans, 2011, Aulonia kratochvili Dunin et al., 1986, Ero flammeola Simon, 1881, Hogna ferox (Lucas, 1838), Maculoncus parvipalpus Wunderlich, 1995, Neon rayi (Simon, 1875), Pardosa aenigmatica Tongiorgi, 1966 and Phyxelida anatolica Griswold, 1990, are illustrated. Tarentula jaffa Strand, 1913, syn. n. is synonymised with Hogna ferox (Lucas, 1838), and Hahnia carmelita Levy, 2007, syn. n. is synonymised with Hahnia nava (Westring, 1851). A possible synonymy of the widespread Prodidomus rufus Hentz, 1847 with P. hispanicus Dalmas, 1919 known from the Iberian Peninsula is discussed. An original combination is restored for Trochosa urbana O.P.-Cambridge, 1876.