Characterization of the VEGF Binding Site on the Flt-1 Receptor
Copyright policy )Characterization of the VEGF Binding Site on the Flt-1 Receptor
The angiogenic growth factor VEGF binds to the receptor tyrosine kinases Flt-1 and KDR/Flk-1. Immunoglobulin (Ig)-like loop-2 of Flt-1 is involved in binding VEGF, but the contribution of other Flt-1 Ig-loops to VEGF binding remains unclear. We tested the ability of membrane-bound chimeras between the extracellular domain of Flt-1 and the cell adhesion molecule embigin to bind VEGF. VEGF bound as well to receptors containing Flt-1 loops 1-2 or 2-3 as it did to the entire Flt-1 extracellular domain. Chimeras containing only loop-2 of Flt-1 bound VEGF with 22-fold lower affinity. We conclude that high-affinity VEGF binding requires Ig-like loop-2 plus either loop-1 or loop-3. In addition, Flt-1 amino acid residues Arg-224 and Asp-231 were not essential for high-affinity binding of VEGF to membrane-bound Flt-1.
- Boston Children's Hospital United States
- American Type Culture Collection United States
- St. Jude Children's Research Hospital United States
Lymphokines, Binding Sites, Membrane Glycoproteins, Base Sequence, Molecular Sequence Data, Membrane Proteins, Receptor Protein-Tyrosine Kinases, Endothelial Growth Factors, Protein Structure, Secondary, Rats, Kinetics, Amino Acid Substitution, Proto-Oncogene Proteins, Mutagenesis, Site-Directed, Animals, Humans, Cloning, Molecular, DNA Primers, Glycoproteins, Molecular Chaperones
Lymphokines, Binding Sites, Membrane Glycoproteins, Base Sequence, Molecular Sequence Data, Membrane Proteins, Receptor Protein-Tyrosine Kinases, Endothelial Growth Factors, Protein Structure, Secondary, Rats, Kinetics, Amino Acid Substitution, Proto-Oncogene Proteins, Mutagenesis, Site-Directed, Animals, Humans, Cloning, Molecular, DNA Primers, Glycoproteins, Molecular Chaperones
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).15 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!