Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter
Copyright policy )Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter
As a novel candidate metastasis suppressor gene, Nasopharyngeal carcinoma-associated gene 6 (NGX6) is involved in cellular growth, cell cycle progression and tumor angiogenesis. Previous studies have shown that NGX6 gene is down-regulated in colorectal cancer (CRC). However, little is known about its transcriptional regulation.We defined the minimal promoter of NGX6 gene in a 186-bp region (from-86 to +100) through mutation construct methods and luciferase assays. Results from Electrophoretic mobility shift assays (EMSA) and Chromatin immunoprecipitation (ChIP) revealed that Early growth response gene 1 (Egr-1) binds to the Sp1/Egr-1 overlapping site of NGX6 minimal promoter. Overexpression of Egr-1 increased the promoter activity and mRNA level of NGX6 gene; while knock-down of endogenous Egr-1 decreased mRNA expression of NGX6 gene.These results demonstrate that Egr-1 regulates NGX6 gene transcription through an overlapping Sp1/Egr-1 binding site as a positive regulator of NGX6 gene.
- Peking University Cancer Hospital China (People's Republic of)
- Central South University China (People's Republic of)
- Hunan Provincial People 's Hospital China (People's Republic of)
- Zhuzhou Central Hospital China (People's Republic of)
- Zhuzhou Central Hospital China (People's Republic of)
Transcriptional Activation, Binding Sites, Base Sequence, Colon, Sp1 Transcription Factor, Tumor Suppressor Proteins, Molecular Sequence Data, Membrane Proteins, Gene Expression Regulation, Neoplastic, Cell Line, Tumor, Colonic Neoplasms, Humans, Promoter Regions, Genetic, Molecular Biology, Research Article, Early Growth Response Protein 1, Protein Binding
Transcriptional Activation, Binding Sites, Base Sequence, Colon, Sp1 Transcription Factor, Tumor Suppressor Proteins, Molecular Sequence Data, Membrane Proteins, Gene Expression Regulation, Neoplastic, Cell Line, Tumor, Colonic Neoplasms, Humans, Promoter Regions, Genetic, Molecular Biology, Research Article, Early Growth Response Protein 1, Protein Binding
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