The amiloride-sensitive epithelial Na+channel (ENaC) is essential for fluid clearance from the airways. An experimental animal model with a reduced expression of ENaC, the α-ENaC transgenic rescue mouse, is prone to develop edema under hypoxia exposure. This strongly suggests an involvement of ENaC in the pathogenesis of pulmonary edema. To investigate the pathogenesis of this type of edema, primary cultures of tracheal cells from these mice were studied in vitro. An ∼60% reduction in baseline amiloride-sensitive Na+transport was observed, but the pharmacological characteristics and physiological regulation of the channel were similar to those observed in cells from wild-type mice. Aprotinin, an inhibitor of serine proteases, blocked 50–60% of the basal transepithelial current, hypoxia induced downregulation of Na+transport, and β-adrenergic stimulation was effective to stimulate Na+transport after the hypoxia-induced decrease. When downregulation of ENaC activity (such as observed under hypoxia) is added to a low “constitutive” ENaC expression, the resulting reduced Na+transport rate may be insufficient for airway fluid clearance and favor pulmonary edema.