Myosin II ATPase Activity Mediates the Long-Term Potentiation-Induced Exodus of Stable F-Actin Bound by Drebrin A from Dendritic Spines
Copyright policy )Myosin II ATPase Activity Mediates the Long-Term Potentiation-Induced Exodus of Stable F-Actin Bound by Drebrin A from Dendritic Spines
The neuronal actin-binding protein drebrin A forms a stable structure with F-actin in dendritic spines. NMDA receptor activation causes an exodus of F-actin bound by drebrin A (DA-actin) from dendritic spines, suggesting a pivotal role for DA-actin exodus in synaptic plasticity. We quantitatively assessed the extent of DA-actin localization to spines using the spine-dendrite ratio of drebrin A in cultured hippocampal neurons, and found that (1) chemical long-term potentiation (LTP) stimulation induces rapid DA-actin exodus and subsequent DA-actin re-entry in dendritic spines, (2) Ca(2+) influx through NMDA receptors regulates the exodus and the basal accumulation of DA-actin, and (3) the DA-actin exodus is blocked by myosin II ATPase inhibitor, but is not blocked by myosin light chain kinase (MLCK) or Rho-associated kinase (ROCK) inhibitors. These results indicate that myosin II mediates the interaction between NMDA receptor activation and DA-actin exodus in LTP induction. Furthermore, myosin II seems to be activated by a rapid actin-linked mechanism rather than slow MLC phosphorylation. Thus the myosin-II mediated DA-actin exodus might be an initial event in LTP induction, triggering actin polymerization and spine enlargement.
Science, Dendritic Spines, Long-Term Potentiation, Glycine, Glutamic Acid, Bicuculline, Hippocampus, Pregnancy, Animals, GABA-A Receptor Antagonists, Phosphorylation, Cells, Cultured, Adenosine Triphosphatases, Myosin Type II, Neurons, Q, Neuropeptides, R, Actins, Microscopy, Fluorescence, Medicine, Calcium, Female, Research Article, Protein Binding
Science, Dendritic Spines, Long-Term Potentiation, Glycine, Glutamic Acid, Bicuculline, Hippocampus, Pregnancy, Animals, GABA-A Receptor Antagonists, Phosphorylation, Cells, Cultured, Adenosine Triphosphatases, Myosin Type II, Neurons, Q, Neuropeptides, R, Actins, Microscopy, Fluorescence, Medicine, Calcium, Female, Research Article, Protein Binding
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