MPK9 and MPK12 function in SA-induced stomatal closure in Arabidopsis thaliana
Copyright policy )MPK9 and MPK12 function in SA-induced stomatal closure in Arabidopsis thaliana
Abstract Salicylic acid (SA) induces stomatal closure sharing several components with abscisic acid (ABA) and methyl jasmonate (MeJA) signaling. We have previously shown that two guard cell-preferential mitogen-activated protein kinases (MAPKs), MPK9 and MPK12, positively regulate ABA signaling and MeJA signaling in Arabidopsis thaliana. In this study, we examined whether these two MAPKs are involved in SA-induced stomatal closure using genetic mutants and a pharmacological, MAPKK inhibitor. Salicylic acid induced stomatal closure in mpk9 and mpk12 single mutants but not in mpk9 mpk12 double mutants. The MAPKK inhibitor PD98059 inhibited SA-induced stomatal closure in wild-type plants. Salicylic acid induced extracellular reactive oxygen species (ROS) production, intracellular ROS accumulation, and cytosolic alkalization in the mpk9, mpk12, and mpk9 mpk12 mutants. Moreover, SA-activated S-type anion channels in guard cells of wild-type plants but not in guard cells of mpk9 mpk12 double mutants. These results imply that MPK9 and MPK12 are positive regulators of SA signaling in Arabidopsis guard cells.
- Okayama University Japan
- Pomona College United States
- Daegu Gyeongbuk Institute of Science and Technology Korea (Republic of)
- Institute for Basic Science Korea (Republic of)
- Claremont Colleges United States
Cytosolic, mitogen-activated protein kinase, Oxylipin, Arabidopsis, Jasmonic Acid Methyl Ester, MPK12 Protein, Acetates, Membrane Potentials, Methyl Jasmonate, Cell Signaling, Plant Growth Regulators, Gene Expression Regulation, Plant, Voltage-Dependent Anion Channels, reactive oxygen species, pH, Oxygen Species Production, cytosolic alkalization, Hydrogen-Ion Concentration, Hydrogen Ion Concentration, S-type anion channel in guard cells, Enzymes, Mitogen Activated Protein Kinase, Hypersensitive Response, Mitogen-Activated Protein Kinases, Salicylic Acid, Activated Protein Kinases, Abscisic Acid (ABA), Guard Cells, Signal Transduction, 570, salicylic acid, Arabidopsis Thaliana, Cells, Mitogen Activated Protein Kinases, Drug Effects, Arabidopsis Protein, Cyclopentanes, Membrane Potential, Enzyme Activity, MPK9 Protein, Genetics, Oxylipins, Protein Kinase Inhibitors, 580, Flavonoids, Arabidopsis Proteins, Cyclopentane Derivative, Acetic Acid Derivative, Plant, Cytosolic Calcium, Metabolism, Gene Expression Regulation, Mutation, Plant Stomata, Flavonoid, Cytology, Reactive Oxygen Species, Agonists, Abscisic Acid
Cytosolic, mitogen-activated protein kinase, Oxylipin, Arabidopsis, Jasmonic Acid Methyl Ester, MPK12 Protein, Acetates, Membrane Potentials, Methyl Jasmonate, Cell Signaling, Plant Growth Regulators, Gene Expression Regulation, Plant, Voltage-Dependent Anion Channels, reactive oxygen species, pH, Oxygen Species Production, cytosolic alkalization, Hydrogen-Ion Concentration, Hydrogen Ion Concentration, S-type anion channel in guard cells, Enzymes, Mitogen Activated Protein Kinase, Hypersensitive Response, Mitogen-Activated Protein Kinases, Salicylic Acid, Activated Protein Kinases, Abscisic Acid (ABA), Guard Cells, Signal Transduction, 570, salicylic acid, Arabidopsis Thaliana, Cells, Mitogen Activated Protein Kinases, Drug Effects, Arabidopsis Protein, Cyclopentanes, Membrane Potential, Enzyme Activity, MPK9 Protein, Genetics, Oxylipins, Protein Kinase Inhibitors, 580, Flavonoids, Arabidopsis Proteins, Cyclopentane Derivative, Acetic Acid Derivative, Plant, Cytosolic Calcium, Metabolism, Gene Expression Regulation, Mutation, Plant Stomata, Flavonoid, Cytology, Reactive Oxygen Species, Agonists, Abscisic Acid
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