Voltage-gated sodium channel (Nav1) β2 subunits modulate channel gating, assembly, and cell-surface expression in CNS neuronsin vitroandin vivo. β2 expression increases in sensory neurons after nerve injury, and development of mechanical allodynia in the spared nerve injury model is attenuated in β2-null mice. Thus, we hypothesized that β2 modulates electrical excitability in dorsal root ganglion (DRG) neuronsin vivo. We compared sodium currents (INa) in small DRG neurons from β2+/+and β2−/−mice to determine the effects of β2 on tetrodotoxin-sensitive (TTX-S) and tetrodotoxin-resistant (TTX-R) Nav1in vivo. Small-fast DRG neurons acutely isolated from β2−/−mice showed significant decreases in TTX-SINacompared with β2+/+neurons. This decrease included a 51% reduction in maximal sodium conductance with no detectable changes in the voltage dependence of activation or inactivation. TTX-S, but not TTX-R, INaactivation and inactivation kinetics in these cells were slower in β2−/−mice compared with controls. The selective regulation of TTX-SINawas supported by reductions in transcript and protein levels of TTX-S Nav1s, particularly Nav1.7. Low-threshold mechanical sensitivity was preserved in β2−/−mice, but they were more sensitive to noxious thermal stimuli than wild type whereas their response during the late phase of the formalin test was attenuated. Our results suggest that β2 modulates TTX-S Nav1 mRNA and protein expression resulting in increased TTX-S INaand increases the rates of TTX-S Nav1 activation and inactivation in small-fast DRG neuronsin vivo. TTX-R INawere not significantly modulated by β2.