Nob1p Is Required for Cleavage of the 3′ End of 18S rRNA
Copyright policy )Nob1p Is Required for Cleavage of the 3′ End of 18S rRNA
We report the characterization of a novel factor, Nob1p (Yor056c), which is essential for the synthesis of 40S ribosome subunits. Genetic depletion of Nob1p strongly inhibits the processing of the 20S pre-rRNA to the mature 18S rRNA, leading to the accumulation of high levels of the 20S pre-rRNA together with novel degradation intermediates. 20S processing occurs within a pre-40S particle after its export from the nucleus to the cytoplasm. Consistent with a direct role in this cleavage, Nob1p was shown to be associated with the pre-40S particle and to be present in both the nucleus and the cytoplasm. This suggests that Nob1p accompanies the pre-40S ribosomes during nuclear export. Pre-40S export is not, however, inhibited by depletion of Nob1p.
- University of Michigan–Ann Arbor United States
- Sapienza University of Rome Italy
- University of Edinburgh United Kingdom
- University of Michigan–Flint United States
- Wellcome Centre for Cell Biology United Kingdom
Cell Nucleus, Cytoplasm, Models, Genetic, Amino Acid Motifs, Molecular Sequence Data, Active Transport, Cell Nucleus, Oligonucleotides, Nuclear Proteins, Blotting, Northern, Protein Structure, Tertiary, Fungal Proteins, Microscopy, Fluorescence, RNA, Ribosomal, Centrifugation, Density Gradient, RNA, Ribosomal, 18S, Amino Acid Sequence, Carrier Proteins, Fluorescent Antibody Technique, Indirect, Ribosomes, Alleles
Cell Nucleus, Cytoplasm, Models, Genetic, Amino Acid Motifs, Molecular Sequence Data, Active Transport, Cell Nucleus, Oligonucleotides, Nuclear Proteins, Blotting, Northern, Protein Structure, Tertiary, Fungal Proteins, Microscopy, Fluorescence, RNA, Ribosomal, Centrifugation, Density Gradient, RNA, Ribosomal, 18S, Amino Acid Sequence, Carrier Proteins, Fluorescent Antibody Technique, Indirect, Ribosomes, Alleles
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