Phenotypic Analysis of Dlx5 Overexpression in Post-natal Bone
Copyright policy )Phenotypic Analysis of Dlx5 Overexpression in Post-natal Bone
Dlx5 plays an important role in the embryonic development of mineralized tissues. We hypothesized that Dlx5 also functions in regulating post-natal bone formation in mice. To prove this hypothesis, we infected 5-day-old bone sialoprotein (BSP)/avian retroviral receptor gene (TVA) transgenic mice with replication-competent retroviral vectors expressing wild-type Dlx5 (RCAS- Dlx5WT) and mutated Dlx5 at arginine (R) 31 of its homeodomain (RCAS- Dlx5RH). Immunohistochemistry indicated that RCAS- Dlx5WT increased BSP and osteopontin (OPN) expression, whereas it decreased that of osteocalcin (OC). RCAS- Dlx5RH mediated opposite effects. Semi-quantitative RT-PCR confirmed these results. Ex vivo overexpression of RCAS- Dlx5WT in BSP/TVA calvarial cells promoted, whereas that of RCAS- Dlx5RH inhibited, mineralized nodule formation as compared with that in control cells. Our results suggest that Dlx5 promotes expression of early markers of osteogenic differentiation and increases mineralization post-natally.
- Shandong Women’s University China (People's Republic of)
- Southern Medical University China (People's Republic of)
- Osaka Gakuin University Japan
- Tufts University United States
Homeodomain Proteins, Extracellular Matrix Proteins, Reverse Transcriptase Polymerase Chain Reaction, Sialoglycoproteins, Genetic Vectors, Osteocalcin, Mice, Transgenic, Arginine, Bone and Bones, Avian Proteins, Mice, Calcification, Physiologic, Phenotype, Gene Expression Regulation, Osteogenesis, Mutation, Animals, Integrin-Binding Sialoprotein, Receptors, Virus, Osteopontin
Homeodomain Proteins, Extracellular Matrix Proteins, Reverse Transcriptase Polymerase Chain Reaction, Sialoglycoproteins, Genetic Vectors, Osteocalcin, Mice, Transgenic, Arginine, Bone and Bones, Avian Proteins, Mice, Calcification, Physiologic, Phenotype, Gene Expression Regulation, Osteogenesis, Mutation, Animals, Integrin-Binding Sialoprotein, Receptors, Virus, Osteopontin
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