Role of hypoxia inducible factors 1α and 2α in basal adhesion formation and in carbon dioxide pneumoperitoneum-enhanced adhesion formation after laparoscopic surgery in transgenic mice
Copyright policy )Role of hypoxia inducible factors 1α and 2α in basal adhesion formation and in carbon dioxide pneumoperitoneum-enhanced adhesion formation after laparoscopic surgery in transgenic mice
To evaluate the role of hypoxia inducible factors (HIFs) 1alpha and 2alpha in adhesion formation after laparoscopic surgery.Prospective, randomized study.Academic research center.Forty Swiss/129SvJ wild-type mice and transgenic mice partially deficient for the genes encoding for HIF-1alpha (HIF-1alpha(+/-)) or HIF-2alpha (HIF-2alpha(+/-)).Adhesions were induced by standardized lesions during laparoscopy. To evaluate "basal adhesions" and "pneumoperitoneum-enhanced adhesions," the pneumoperitoneum was maintained for a minimum (10 minutes) or prolonged (60 minutes) period, respectively.Adhesions were blindly scored after 7 days.In both HIF-1alpha and HIF-2alpha wild-type mice, pneumoperitoneum enhanced adhesion formation. In comparison with wild-type mice, basal adhesions were lower in HIF-1alpha(+/-) and similar in HIF-2alpha(+/-) mice. Pneumoperitoneum did not enhance adhesion formation in HIF-1alpha(+/-) or in HIF-2alpha(+/-) mice. Therefore, in comparison with the correspondent wild-type mice, pneumoperitoneum-enhanced adhesions were lower in HIF-1alpha(+/-) and HIF-2alpha(+/-) mice.These data confirm that CO(2) pneumoperitoneum enhances adhesion formation and indicate that this effect is mediated, at least in part, by an up-regulation of HIF-1alpha and HIF-2alpha.
- Katholieke Universiteit Leuven Belgium
- KU Leuven Belgium
- Leuven Institute for Fertility and Embryology Belgium
Nuclear Proteins, Mice, Transgenic, Tissue Adhesions, Carbon Dioxide, Hypoxia-Inducible Factor 1, alpha Subunit, DNA-Binding Proteins, Mice, Random Allocation, Postoperative Complications, Basic Helix-Loop-Helix Transcription Factors, Trans-Activators, Animals, Female, Laparoscopy, Hypoxia-Inducible Factor 1, Prospective Studies, Peritoneum, Pneumoperitoneum, Artificial, Transcription Factors
Nuclear Proteins, Mice, Transgenic, Tissue Adhesions, Carbon Dioxide, Hypoxia-Inducible Factor 1, alpha Subunit, DNA-Binding Proteins, Mice, Random Allocation, Postoperative Complications, Basic Helix-Loop-Helix Transcription Factors, Trans-Activators, Animals, Female, Laparoscopy, Hypoxia-Inducible Factor 1, Prospective Studies, Peritoneum, Pneumoperitoneum, Artificial, Transcription Factors
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