TheArabidopsis thalianaHomolog of YeastBRE1Has a Function in Cell Cycle Regulation during Early Leaf and Root Growth
Copyright policy )TheArabidopsis thalianaHomolog of YeastBRE1Has a Function in Cell Cycle Regulation during Early Leaf and Root Growth
AbstractChromatin modification and transcriptional activation are novel roles for E3 ubiquitin ligase proteins that have been mainly associated with ubiquitin-dependent proteolysis. We identified HISTONE MONOUBIQUITINATION1 (HUB1) (and its homolog HUB2) in Arabidopsis thaliana as RING E3 ligase proteins with a function in organ growth. We show that HUB1 is a functional homolog of the human and yeast BRE1 proteins because it monoubiquitinated histone H2B in an in vitro assay. Hub knockdown mutants had pale leaf coloration, modified leaf shape, reduced rosette biomass, and inhibited primary root growth. One of the alleles had been designated previously as ang4-1. Kinematic analysis of leaf and root growth together with flow cytometry revealed defects in cell cycle activities. The hub1-1 (ang4-1) mutation increased cell cycle duration in young leaves and caused an early entry into the endocycles. Transcript profiling of shoot apical tissues of hub1-1 (ang4-1) indicated that key regulators of the G2-to-M transition were misexpressed. Based on the mutant characterization, we postulate that HUB1 mediates gene activation and cell cycle regulation probably through chromatin modifications.
- Miguel Hernandez University Spain
- Vlaams Instituut voor Biotechnologie Belgium
- Ghent University Belgium
- University of Adelaide Australia
Transcriptional Activation, Saccharomyces cerevisiae Proteins, Ubiquitin-Protein Ligases, Molecular Sequence Data, ORGAN GROWTH, RNA-POLYMERASE-II, Arabidopsis, PROTEIN, Plant Roots, UBIQUITIN, Ligases, MEDIATED TRANSFORMATION, Gene Expression Regulation, Plant, Humans, HISTONE H2B MONOUBIQUITINATION, GENE-EXPRESSION, Cell Proliferation, Oligonucleotide Array Sequence Analysis, Arabidopsis Proteins, Ubiquitin, Gene Expression Profiling, PLANT DEVELOPMENT, Cell Cycle, Biology and Life Sciences, Ubiquitin-Protein Ligase Complexes, SIZE CONTROL, Plant, MUTANTS IDENTIFY, Plant Leaves, Phenotype, Gene Expression Regulation
Transcriptional Activation, Saccharomyces cerevisiae Proteins, Ubiquitin-Protein Ligases, Molecular Sequence Data, ORGAN GROWTH, RNA-POLYMERASE-II, Arabidopsis, PROTEIN, Plant Roots, UBIQUITIN, Ligases, MEDIATED TRANSFORMATION, Gene Expression Regulation, Plant, Humans, HISTONE H2B MONOUBIQUITINATION, GENE-EXPRESSION, Cell Proliferation, Oligonucleotide Array Sequence Analysis, Arabidopsis Proteins, Ubiquitin, Gene Expression Profiling, PLANT DEVELOPMENT, Cell Cycle, Biology and Life Sciences, Ubiquitin-Protein Ligase Complexes, SIZE CONTROL, Plant, MUTANTS IDENTIFY, Plant Leaves, Phenotype, Gene Expression Regulation
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).152 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!