Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.
Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.
A cDNA encoding the CD2 antigen has been isolated by a highly efficient technique based on transient expression in COS cells and adherence of cells expressing surface antigen to antibody-coated dishes. COS cells expressing a CD2 cDNA isolated by this method readily formed rosettes with sheep as well as human and other mammalian erythrocytes. Pretreatment of transfected COS cells with anti-CD2 antibody, or pretreatment of human erythrocytes with anti-LFA-3 antibody, abolished rosette formation.
Antigens, Differentiation, T-Lymphocyte, Erythrocytes, Base Sequence, T-Lymphocytes, Genetic Vectors, DNA, Cell Line, Epitopes, Antigens, Surface, Animals, Humans, Amino Acid Sequence, Cloning, Molecular
Antigens, Differentiation, T-Lymphocyte, Erythrocytes, Base Sequence, T-Lymphocytes, Genetic Vectors, DNA, Cell Line, Epitopes, Antigens, Surface, Animals, Humans, Amino Acid Sequence, Cloning, Molecular
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