Impaired Angiogenic Response in the Corneas of Mice Lacking Osteopontin
Copyright policy )Impaired Angiogenic Response in the Corneas of Mice Lacking Osteopontin
To investigate the effects of loss of osteopontin (OPN) in the development of neovascularization in corneal stroma in mice. Cell culture study was also conducted to clarify the effects of OPN in transforming growth factor (TGF) beta1-driven cell signaling and expression of vascular endothelial growth factor (VEGF).Ocular fibroblasts from wild-type and OPN-null mice were used to study the role of OPN in TGFbeta1 signal and VEGF expression. The effect of the absence of OPN on corneal neovascularization was evaluated in mice.In ocular fibroblast culture, loss of OPN attenuated TGFbeta1 signals (Smad3 and p38) and reduced expression of VEGF. Loss of OPN attenuated neovascularization in corneal stroma in mice.OPN is involved in VEGF expression in cultured fibroblasts and is required for neovascularization in corneal stroma in vivo.
- Rutgers, The State University of New Jersey United States
- Hokkaido Bunkyo University Japan
- Hokkaido University Japan
- Wakayama Medical University Japan
Mice, Knockout, Vascular Endothelial Growth Factor A, Reverse Transcriptase Polymerase Chain Reaction, Corneal Stroma, Fibroblasts, Up-Regulation, Cornea, Mice, Inbred C57BL, Platelet Endothelial Cell Adhesion Molecule-1, Transforming Growth Factor beta1, Mice, Animals, Newborn, Macrophages, Peritoneal, Animals, Corneal Neovascularization, Osteopontin, RNA, Messenger, Cells, Cultured, Signal Transduction
Mice, Knockout, Vascular Endothelial Growth Factor A, Reverse Transcriptase Polymerase Chain Reaction, Corneal Stroma, Fibroblasts, Up-Regulation, Cornea, Mice, Inbred C57BL, Platelet Endothelial Cell Adhesion Molecule-1, Transforming Growth Factor beta1, Mice, Animals, Newborn, Macrophages, Peritoneal, Animals, Corneal Neovascularization, Osteopontin, RNA, Messenger, Cells, Cultured, Signal Transduction
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