Solution Structure of the Major DNA-binding Domain of Arabidopsis thaliana Ethylene-insensitive3-like3
Copyright policy )Solution Structure of the Major DNA-binding Domain of Arabidopsis thaliana Ethylene-insensitive3-like3
Ethylene-insensitive3 (EIN3) and EIN3-like (EIL) proteins are essential transcription factors in the ethylene signaling of higher plants. The EIN3/EIL proteins bind to the promoter regions of the downstream genes and regulate their expression. The location of the DNA-binding domain (DBD) in the primary structure was unclear, since the proteins show no sequence similarity to other known DBDs. Here, we identify the major DBD of an EIN3/EIL protein, Arabidopsis thaliana EIL3, containing a key mutational site for DNA binding and signaling (ein3-3 site), and determine its solution structure by NMR spectroscopy. The structure consists of five alpha-helices, possessing a novel fold dissimilar to known DBD structures. By a chemical-shift perturbation analysis, a region including the ein3-3 site is suggested to be involved in DNA binding.
Models, Molecular, Proline, Arabidopsis Proteins, Molecular Sequence Data, Arabidopsis, Nuclear Proteins, DNA, Surface Plasmon Resonance, Protein Structure, Tertiary, DNA-Binding Proteins, Structural Homology, Protein, Amino Acid Sequence, Nuclear Magnetic Resonance, Biomolecular, Sequence Alignment, Transcription Factors
Models, Molecular, Proline, Arabidopsis Proteins, Molecular Sequence Data, Arabidopsis, Nuclear Proteins, DNA, Surface Plasmon Resonance, Protein Structure, Tertiary, DNA-Binding Proteins, Structural Homology, Protein, Amino Acid Sequence, Nuclear Magnetic Resonance, Biomolecular, Sequence Alignment, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).78 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!