Loading of an Mcm Protein onto DNA Replication Origins Is Regulated by Cdc6p and CDKs
Copyright policy )Loading of an Mcm Protein onto DNA Replication Origins Is Regulated by Cdc6p and CDKs
In eukaryotic cells, firing of DNA replication origins normally does not recur until after M phase. This characteristic is thought to be due to the properties of "initiation" proteins like Orc, Cdc6, and Mcms. Using formaldehyde cross-linking, we show that Cdc6p and Mcm7p associate specifically with replication origins during G1 but not during G2 in S. cerevisiae. Mcm7p's association with origins depends on Cdc6p. Ectopic expression of Cdc6p enables it to associate with origins during G2, but this fails to recruit Mcm7p. Our data suggest that the loading of Mcm proteins onto origins is regulated by two mechanisms: first, by Cdc6p occupancy, and second, by S- and M-CDKs, whose activity during S, G2, and M phases prevents Mcm loading.
DNA Replication, G2 Phase, Binding Sites, Saccharomyces cerevisiae Proteins, Biochemistry, Genetics and Molecular Biology(all), G1 Phase, Origin Recognition Complex, Mitosis, Nuclear Proteins, Cell Cycle Proteins, Saccharomyces cerevisiae, Minichromosome Maintenance Complex Component 7, Cyclin-Dependent Kinases, DNA-Binding Proteins, Fungal Proteins, Protein Binding
DNA Replication, G2 Phase, Binding Sites, Saccharomyces cerevisiae Proteins, Biochemistry, Genetics and Molecular Biology(all), G1 Phase, Origin Recognition Complex, Mitosis, Nuclear Proteins, Cell Cycle Proteins, Saccharomyces cerevisiae, Minichromosome Maintenance Complex Component 7, Cyclin-Dependent Kinases, DNA-Binding Proteins, Fungal Proteins, Protein Binding
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).468 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 0.1%
Citations provided by BIP!Popularity provided by BIP!