Lower and upper stem–single-stranded RNA junctions together determine the Drosha cleavage site
Copyright policy )Lower and upper stem–single-stranded RNA junctions together determine the Drosha cleavage site
Significance MicroRNA genes are transcribed as long primary microRNAs (pri-miRNAs) and then processed by the Drosha–DGCR8 (DiGeorge syndrome critical region gene 8) complex into ∼60-nt pre-miRNAs, which is the key step of miRNA biogenesis. How exactly the Drosha–DGCR8 complex recognizes pri-miRNAs and selects the processing site is not well understood. Here we developed a unique approach to examining the details of how this complex chooses the processing site in human cells. Our study yields new knowledge of how the Drosha–DGCR8 complex determines its processing sites and also provides an explanation for how 5′ isomiRs are generated in some miRNAs, which is another interesting but not clearly understood phenomenon in miRNA biogenesis.
- The University of Texas System United States
- Texas Tech University Health Sciences Center United States
- Texas Tech University United States
Ribonuclease III, MicroRNAs, Humans, Nucleic Acid Conformation, RNA Processing, Post-Transcriptional, Cell Line
Ribonuclease III, MicroRNAs, Humans, Nucleic Acid Conformation, RNA Processing, Post-Transcriptional, Cell Line
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).92 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!