Accurate Rh phenotype determination by reticulocyte mRNA typing shortly after multiple transfusions
Copyright policy )Accurate Rh phenotype determination by reticulocyte mRNA typing shortly after multiple transfusions
Alloimmunization is a common phenomenon after transfusion, with an estimated incidence of 0.5% increasing to 20-60% in chronically transfused patients. In recently transfused patients, serological typing can be hampered by mixed field agglutination. We established RT-PCR methods for RHD, RHC/c and RHE/e typing using mRNA from reticulocytes. Molecular typing was performed soon after 51 separate mismatched transfusion events involving 30 patients. Accurate identification of the transfused patients' phenotype was confirmed in all cases. Reticulocyte maturation studies revealed that temperature is a crucial parameter for transition into mature red blood cells.
- University of Oslo Norway
Adult, Aged, 80 and over, Male, DNA, Complementary, Membrane Glycoproteins, Adolescent, Cell Survival, Hemagglutination Tests, Middle Aged, Cold Temperature, Phenotype, Blood Grouping and Crossmatching, Humans, Diseases of the blood and blood-forming organs, Erythropoiesis, Female, RC633-647.5, Leukocyte Reduction Procedures, Artifacts, Erythrocyte Transfusion, Cation Transport Proteins, Aged
Adult, Aged, 80 and over, Male, DNA, Complementary, Membrane Glycoproteins, Adolescent, Cell Survival, Hemagglutination Tests, Middle Aged, Cold Temperature, Phenotype, Blood Grouping and Crossmatching, Humans, Diseases of the blood and blood-forming organs, Erythropoiesis, Female, RC633-647.5, Leukocyte Reduction Procedures, Artifacts, Erythrocyte Transfusion, Cation Transport Proteins, Aged
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).4 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!