Enhanced Inducible Costimulator Ligand (ICOS-L) Expression on Dendritic Cells in Interleukin-10 Deficiency and Its Impact on T-Cell Subsets in Respiratory Tract Infection
Copyright policy )Enhanced Inducible Costimulator Ligand (ICOS-L) Expression on Dendritic Cells in Interleukin-10 Deficiency and Its Impact on T-Cell Subsets in Respiratory Tract Infection
AbstractAn association between inducible costimulator ligand (ICOS-L) expression and interleukin (IL)-10 production by dendritic cells (DCs) has been commonly found in infectious disease. DCs with higher ICOS-L expression and IL-10 production are reportedly more efficient in inducing regulatory T cells (Tregs). Here we use the Chlamydia muridarum(Cm) lung infection model in IL-10 knockout (KO) mice to test the relationship between IL-10 production and ICOS-L expression by DCs. We examined ICOS-L expression, the development of T-cell subsets, including Treg, Th17 and Th1 cell, in the background of IL-10 deficiency and its relationship with ICOS-L/ICOS signaling after infection. Surprisingly, we found that the IL-10 KO mice exhibited significantly higher ICOS-L expression by DCs. Moreover, IL-10 KO mice showed lower Tregs but higher Th17 and Th1 responses, but only the Th17 response depended on ICOS signaling. Consistently, most of the Th17 cells were ICOS+, whereas most of the Th1 cells were ICOS− in the infected mice. Furthermore, neutralization of IL-17 in IL-10 KO mice significantly exacerbated lung infection. The data suggest that ICOS-L expression on DC may be negatively regulated by IL-10 and that ICOS-L expression on DC in the presence or absence of IL-10 costimulation may promote Treg or Th17 response, without significant impact on Th1.
- University of Manitoba Canada
Chlamydia muridarum, RM1-950, QD415-436, Biochemistry, T-Lymphocytes, Regulatory, Inducible T-Cell Co-Stimulator Ligand, Mice, T-Lymphocyte Subsets, Animals, Humans, Lung, Respiratory Tract Infections, Mice, Knockout, Interleukin-17, Dendritic Cells, Chlamydia Infections, Th1 Cells, Inducible Costimulator (ICOS), ICOS Signaling, Low Treg, Interleukin-10, Mice, Inbred C57BL, Gene Expression Regulation, Chlamydia Muridarum, Th17 Cells, Female, Therapeutics. Pharmacology, Th1 Response, HeLa Cells
Chlamydia muridarum, RM1-950, QD415-436, Biochemistry, T-Lymphocytes, Regulatory, Inducible T-Cell Co-Stimulator Ligand, Mice, T-Lymphocyte Subsets, Animals, Humans, Lung, Respiratory Tract Infections, Mice, Knockout, Interleukin-17, Dendritic Cells, Chlamydia Infections, Th1 Cells, Inducible Costimulator (ICOS), ICOS Signaling, Low Treg, Interleukin-10, Mice, Inbred C57BL, Gene Expression Regulation, Chlamydia Muridarum, Th17 Cells, Female, Therapeutics. Pharmacology, Th1 Response, HeLa Cells
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