Altered proliferative ability of neuronal progenitors in PlexinA1 mutant mice
Copyright policy )Altered proliferative ability of neuronal progenitors in PlexinA1 mutant mice
ABSTRACTCortical interneurons are generated predominantly in the medial ganglionic eminence (MGE) and migrate through the ventral and dorsal telencephalon before taking their final positions within the developing cortical plate. Previously we demonstrated that interneurons from Robo1 knockout (Robo1−/−) mice contain reduced levels of neuropilin 1 (Nrp1) and PlexinA1 receptors, rendering them less responsive to the chemorepulsive actions of semaphorin ligands expressed in the striatum and affecting their course of migration (Hernandez‐Miranda et al. [2011] J. Neurosci. 31:6174–6187). Earlier studies have highlighted the importance of Nrp1 and Nrp2 in interneuron migration, and here we assess the role of PlexinA1 in this process. We observed significantly fewer cells expressing the interneuron markersGad67andLhx6in the cortex ofPlexinA1−/−mice compared with wild‐type littermates at E14.5 and E18.5. Although the level of apoptosis was similar in the mutant and control forebrain, proliferation was significantly reduced in the former. Furthermore, progenitor cells in the MGE ofPlexinA1−/−mice appeared to be poorly anchored to the ventricular surface and showed reduced adhesive properties, which may account for the observed reduction in proliferation. Together our data uncover a novel role for PlexinA1 in forebrain development. J. Comp. Neurol. 524:518–534, 2016. © 2015 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.
- University of Melbourne Australia
- UNIVERSITY COLLEGE LONDON, Bartlett School of Planning United Kingdom
- Wellcome Trust United Kingdom
- Kumamoto University Japan
- Institute of Ophthalmology University College London United Kingdom
570, Plexin, forebrain, proliferation, Neurogenesis, Green Fluorescent Proteins, LIM-Homeodomain Proteins, 610, Apoptosis, Nerve Tissue Proteins, Receptors, Cell Surface, Neural Stem Cells, Cell Movement, Interneurons, Cell Adhesion, Animals, Research Articles, Cells, Cultured, In Situ Hybridization, Cell Proliferation, Cerebral Cortex, Mice, Knockout, neuronal migration, interneurons, Glutamate Decarboxylase, Immunohistochemistry, Mice, Inbred C57BL, Transcription Factors
570, Plexin, forebrain, proliferation, Neurogenesis, Green Fluorescent Proteins, LIM-Homeodomain Proteins, 610, Apoptosis, Nerve Tissue Proteins, Receptors, Cell Surface, Neural Stem Cells, Cell Movement, Interneurons, Cell Adhesion, Animals, Research Articles, Cells, Cultured, In Situ Hybridization, Cell Proliferation, Cerebral Cortex, Mice, Knockout, neuronal migration, interneurons, Glutamate Decarboxylase, Immunohistochemistry, Mice, Inbred C57BL, Transcription Factors
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