The effect of moesin overexpression on ageing of human dermal microvascular endothelial cells
Copyright policy )The effect of moesin overexpression on ageing of human dermal microvascular endothelial cells
Abstract: Senescence of microvascular endothelial cells is known to play an important role in the pathophysiology of vascular diseases related to ageing, but the accurate mechanism or related genes are not known. Moesin, a cytoskeletal protein and the most potent candidate as an ageing‐related protein, showed obvious changes in expression when compared before and after ageing. In this study, a lentivirus was used to overexpress moesin in endothelial cells. The expression of cell cycle mediators such as p16, cyclin D1 and cdk4, which can be the markers of ageing, was compared by RNA and was shown to be suppressed in moesin overexpressed endothelial cells. In conclusion, it can be said that the expression of moesin delays senescence of human dermal microvascular endothelial cells and this fundamental discovery can be used as a basis for understanding the mechanism of ageing and age‐related diseases.
- Yonsei University Health System Korea (Republic of)
- Yonsei University Korea (Republic of)
- Yonsei University Medical Library Korea (Republic of)
Microfilament Proteins/biosynthesis*, Aging, Cells, 610, Microfilament Proteins/metabolism, Messenger/metabolism, Fluorescence/methods, Models, Biological, Models, Phase-Contrast/methods, Gene Expression Regulation*, Humans, Microscopy, Phase-Contrast, Endothelium, RNA, Messenger, moesin, Cells, Cultured, Cellular Senescence, Cytoskeleton, Skin, Microscopy, Cultured, Vascular/metabolism*, Microcirculation, Cell Cycle, Microfilament Proteins, Vascular/cytology, Biological, Skin/metabolism*, Gene Expression Regulation, Microscopy, Fluorescence, ageing, Cytoskeleton/metabolism, endothelial cell, RNA, Endothelium, Vascular, overexpression
Microfilament Proteins/biosynthesis*, Aging, Cells, 610, Microfilament Proteins/metabolism, Messenger/metabolism, Fluorescence/methods, Models, Biological, Models, Phase-Contrast/methods, Gene Expression Regulation*, Humans, Microscopy, Phase-Contrast, Endothelium, RNA, Messenger, moesin, Cells, Cultured, Cellular Senescence, Cytoskeleton, Skin, Microscopy, Cultured, Vascular/metabolism*, Microcirculation, Cell Cycle, Microfilament Proteins, Vascular/cytology, Biological, Skin/metabolism*, Gene Expression Regulation, Microscopy, Fluorescence, ageing, Cytoskeleton/metabolism, endothelial cell, RNA, Endothelium, Vascular, overexpression
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