Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region
Copyright policy )Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region
The human kallikrein gene cluster, located in the chromosome band 19q13, contains several tissue-specific serine protease genes including the prostate-specific KLK2, KLK3 and prostase genes. To further characterize the gene cluster, we have mapped, sequenced, and analyzed the genomic sequence from the region. The results of EST database searches and GENSCAN gene prediction analysis reveal 13 serine protease genes and several pseudogenes in the region. Expression analysis by RT-PCR indicates that most of these protease genes are expressed only in a subset of the 35 different normal tissues that have been examined. Several protease genes expressed in skin show higher expression levels in psoriatic lesion samples than in non-lesional skin samples from the same patient. This suggests that the imbalance of a complex protease cascade in skin may contribute to the pathology of disease. The proteases, excluding the kallikrein genes, share approximately 40% of their sequences suggesting that the serine protease gene cluster on chromosome 19q13 arose from ancient gene duplications.
Male, Ovarian Neoplasms, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Chromosome Mapping, DNA, Sequence Analysis, DNA, Gene Expression Regulation, Enzymologic, Evolution, Molecular, Blotting, Southern, Multigene Family, Humans, Psoriasis, RNA, Female, Kallikreins, Amino Acid Sequence, Chromosomes, Human, Pair 19, Sequence Alignment
Male, Ovarian Neoplasms, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Chromosome Mapping, DNA, Sequence Analysis, DNA, Gene Expression Regulation, Enzymologic, Evolution, Molecular, Blotting, Southern, Multigene Family, Humans, Psoriasis, RNA, Female, Kallikreins, Amino Acid Sequence, Chromosomes, Human, Pair 19, Sequence Alignment
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).94 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!