A transposon‐based activation‐tagging population in Arabidopsis thaliana (TAMARA) and its application in the identification of dominant developmental and metabolic mutations
Copyright policy )A transposon‐based activation‐tagging population in Arabidopsis thaliana (TAMARA) and its application in the identification of dominant developmental and metabolic mutations
A population of 9471 stable activation‐tagged lines was generated by transposable element mediated activation tagging mutagenesis in Arabidopsis (TAMARA) using the maize En/Spm transposon system. Based on DNA gel blot and flanking sequence analysis, this population contains approximately 6000 independent transposon insertions. A greenhouse‐based screen identified six dominant or semi‐dominant activation tagged mutants with obvious developmental alterations, among these a new pistillata mutant allele. In addition, a subset of 1500 lines was screened by a HPLC based high‐throughput method for dominant activation tagged mutants with enhanced contents of phenolic compounds. One dominant activation tagged mutant (hpc1‐1D) was isolated showing accumulation of a particular compound due to the upregulation of an R2R3‐MYB transcription factor.
Activation tagging, Dominant mutation, Genetic Markers, DNA Mutational Analysis, Molecular Sequence Data, Arabidopsis, Shoot development, Chromosomes, Plant, Gene Expression Regulation, Mutagenesis, Phenylpropanoid metabolism, DNA Transposable Elements, Amino Acid Sequence, Transposon, Sequence Alignment, Sequence Tagged Sites
Activation tagging, Dominant mutation, Genetic Markers, DNA Mutational Analysis, Molecular Sequence Data, Arabidopsis, Shoot development, Chromosomes, Plant, Gene Expression Regulation, Mutagenesis, Phenylpropanoid metabolism, DNA Transposable Elements, Amino Acid Sequence, Transposon, Sequence Alignment, Sequence Tagged Sites
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