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Molecular Cell
Article
License: Elsevier Non-Commercial
Data sources: UnpayWall
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Molecular Cell
Article . 2003
License: Elsevier Non-Commercial
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Molecular Cell
Article . 2003 . Peer-reviewed
License: Elsevier Non-Commercial
Data sources: Crossref
Molecular Cell
Article . 2003
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Independent Recruitment In Vivo by Gal4 of Two Complexes Required for Transcription

Authors: Bryant, Gene O.; Ptashne, Mark;

Independent Recruitment In Vivo by Gal4 of Two Complexes Required for Transcription

Abstract

We use a modified form of ChIP to analyze the recruitment of seven sets of proteins to the yeast GAL genes upon induction. We resolve three stages of recruitment: first SAGA, then Mediator, and finally Pol II along with four other proteins (including TBP) bind the promoter. In a strain lacking SAGA, Mediator is recruited with a time course indistinguishable from that observed in wild-type cells. Our results are consistent with the notion that a single species of activator, Gal4, separately contacts, and thereby directly recruits, SAGA and Mediator.

Related Organizations
Keywords

Transcriptional Activation, Binding Sites, Mediator Complex, Saccharomyces cerevisiae Proteins, Macromolecular Substances, Cell Biology, DNA-Binding Proteins, Gene Expression Regulation, Gene Expression Regulation, Fungal, Yeasts, Genes, Regulator, Reaction Time, Trans-Activators, RNA Polymerase II, Promoter Regions, Genetic, Molecular Biology, Cells, Cultured, Transcription Factors

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    citations
    This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    156
    popularity
    This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
    Top 10%
    influence
    This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
    Top 1%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Top 1%
Powered by OpenAIRE graph
citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
156
Top 10%
Top 1%
Top 1%
hybrid