Independent Recruitment In Vivo by Gal4 of Two Complexes Required for Transcription
pmid: 12769853
Independent Recruitment In Vivo by Gal4 of Two Complexes Required for Transcription
We use a modified form of ChIP to analyze the recruitment of seven sets of proteins to the yeast GAL genes upon induction. We resolve three stages of recruitment: first SAGA, then Mediator, and finally Pol II along with four other proteins (including TBP) bind the promoter. In a strain lacking SAGA, Mediator is recruited with a time course indistinguishable from that observed in wild-type cells. Our results are consistent with the notion that a single species of activator, Gal4, separately contacts, and thereby directly recruits, SAGA and Mediator.
- Kettering University United States
Transcriptional Activation, Binding Sites, Mediator Complex, Saccharomyces cerevisiae Proteins, Macromolecular Substances, Cell Biology, DNA-Binding Proteins, Gene Expression Regulation, Gene Expression Regulation, Fungal, Yeasts, Genes, Regulator, Reaction Time, Trans-Activators, RNA Polymerase II, Promoter Regions, Genetic, Molecular Biology, Cells, Cultured, Transcription Factors
Transcriptional Activation, Binding Sites, Mediator Complex, Saccharomyces cerevisiae Proteins, Macromolecular Substances, Cell Biology, DNA-Binding Proteins, Gene Expression Regulation, Gene Expression Regulation, Fungal, Yeasts, Genes, Regulator, Reaction Time, Trans-Activators, RNA Polymerase II, Promoter Regions, Genetic, Molecular Biology, Cells, Cultured, Transcription Factors
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