Alteration of glucose metabolism in cultured astrocytes after AQP9-small interference RNA application
Copyright policy )Alteration of glucose metabolism in cultured astrocytes after AQP9-small interference RNA application
Aquaglyceroporin-9 (AQP9) facilitates diffusion of water and energy substrates such as glycerol and monocarboxylates. AQP9 is present in plasma membrane and mitochondria of astrocytes and catecholaminergic neurons, suggesting that it plays a role in the energetic status of these cells. Using specific small interference RNA directed against AQP9 in astrocyte cultures, we showed that glycerol uptake is decreased which is associated with an increase in glucose uptake and oxidative metabolism. Our results not only confirm the presence of AQP9 in astrocytes but also suggest that changes in AQP9 expression alter glial energy metabolism.
- Utrecht University Netherlands
- University Medical Center Utrecht Netherlands
- Loma Linda University United States
- University Hospital of Lausanne Switzerland
- University of Zurich Switzerland
Blotting, Western, 2800 General Neuroscience, 610 Medicine & health, Aquaporins, 1309 Developmental Biology, 10180 Clinic for Neurosurgery, Mice, 2728 Neurology (clinical), Glucose, Astrocytes, Gene Knockdown Techniques, 1312 Molecular Biology, Animals, RNA, Small Interfering, Energy Metabolism, Cells, Cultured
Blotting, Western, 2800 General Neuroscience, 610 Medicine & health, Aquaporins, 1309 Developmental Biology, 10180 Clinic for Neurosurgery, Mice, 2728 Neurology (clinical), Glucose, Astrocytes, Gene Knockdown Techniques, 1312 Molecular Biology, Animals, RNA, Small Interfering, Energy Metabolism, Cells, Cultured
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