Carbohydrate moieties in human secretory component
Copyright policy )Carbohydrate moieties in human secretory component
Human secretory component has seven putative sites for N-linked glycosylation. From tryptic and Glu-C digests we have isolated peptides encompassing asparagines 65, 72, 117, 168, 403, 451 and 481. Analysis by on line HPLC-electrospray mass spectrometry indicated that these residues were fully glycosylated and that the major carbohydrate moieties were far less diversified in composition than expected. Fast atom bombardment mass spectrometry performed on oligosaccharides released by peptide-N-glycosidase F treatment of fractionated and unfractionated SC digests showed the following glycan compositions: Fuc(2)Hex(5)HexNAc(4), Fuc(3)Hex(5)HexNAc(4), NeuAcFucHex(5)HexNAc(4), NeuAcFuc(2)Hex(5)HexNAc(4), NeuAc(2)Hex(5)HexNAc4 and NeuAc(2)FucHex(5)HexNAc(4). Three of these oligosaccharides are the major carbohydrate moieties in human lactoferrin. A possible biological role of the secretory component glycans in the protection of mucosal surfaces is discussed.
- University of Geneva Switzerland
Molecular Sequence Data, Serine Endopeptidases, Carbohydrates, Glycopeptides, Oligosaccharides, Peptide Mapping, Mass Spectrometry, Secretory Component, Carbohydrate Sequence, Humans, Trypsin, Amino Acid Sequence, Chromatography, High Pressure Liquid
Molecular Sequence Data, Serine Endopeptidases, Carbohydrates, Glycopeptides, Oligosaccharides, Peptide Mapping, Mass Spectrometry, Secretory Component, Carbohydrate Sequence, Humans, Trypsin, Amino Acid Sequence, Chromatography, High Pressure Liquid
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).45 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!