Additional file 5: Figure S5. Open field testing. A) No significant interaction was identified by mixed ANOVA between TDP-43M337V and doxycycline (DOX) reduction over time on cumulative distance traveled, (n ≥ 5/group, p = 0.161). Similarly, there were no main effects of time (p = 0.421) or genotype (p = 0.502) on cumulative distance traveled. B) Within ChAT-tTA/TRE-TDP-43M337V rats, there were no significant main effects of time (p = 0.824), tau injection groups (p = 0.085), or the interaction between the two on cumulative distance traveled (n ≥ 3/group, p = 0.300). C) Mixed ANOVA revealed a significant interaction between TDP-43M337V and DOX reduction over time on thigmotaxis, (n ≥ 4/group, p = 0.022). The main effect of time also showed a statistically significant difference in mean thigmotaxis at the different time points, (p = 0.039), however post hoc t-tests with Bonferroni correction revealed no significant differences between any of the time points (p ≥ 0.05). There was also a significant difference in mean thigmotaxis between ChAT-tTA/TRE-TDP-43M337V and TDP-43 controls (p = 0.039) regardless of the time, suggesting that control animal generally spent more time in the perimeter as opposed to the centre of the open-field (* p ≤ 0.05). D) Within ChAT-tTA/TRE-TDP-43M337V rats, there was no significant interaction between tau and time on thigmotaxis, (n ≥ 3/group, p = 0.240). Main effect of time showed a statistically significant difference in mean thigmotaxis at the different time points (p = 0.001) and post hoc t-tests with Bonferroni correction revealed a significant difference between baseline (before DOX reduction) and 2 weeks following DOX reduction (p = 0.004). There was no statistically significant difference in mean thigmotaxis between injection groups (p = 0.383). GFP = green fluorescent protein, WT-tau = GFP-tagged wild-type human tau, Thr175Asp-tau = GFP-tagged Thr175Asp human tau. *p ≤ 0.05.