Lis1 and doublecortin function with dynein to mediate coupling of the nucleus to the centrosome in neuronal migration
Copyright policy )Lis1 and doublecortin function with dynein to mediate coupling of the nucleus to the centrosome in neuronal migration
Humans with mutations in either DCX or LIS1 display nearly identical neuronal migration defects, known as lissencephaly. To define subcellular mechanisms, we have combined in vitro neuronal migration assays with retroviral transduction. Overexpression of wild-type Dcx or Lis1, but not patient-related mutant versions, increased migration rates. Dcx overexpression rescued the migration defect in Lis1+/− neurons. Lis1 localized predominantly to the centrosome, and after disruption of microtubules, redistributed to the perinuclear region. Dcx outlined microtubules extending from the perinuclear “cage” to the centrosome. Lis1+/− neurons displayed increased and more variable separation between the nucleus and the preceding centrosome during migration. Dynein inhibition resulted in similar defects in both nucleus–centrosome (N-C) coupling and neuronal migration. These N-C coupling defects were rescued by Dcx overexpression, and Dcx was found to complex with dynein. These data indicate Lis1 and Dcx function with dynein to mediate N-C coupling during migration, and suggest defects in this coupling may contribute to migration defects in lissencephaly.
- University of California System United States
- University of California, San Diego United States
- UNIVERSITY OF CALIFORNIA SAN DIEGO
Doublecortin Domain Proteins, Doublecortin Protein, Macromolecular Substances, migration, Nervous System Malformations, Microtubules, Article, Lis 1, Mice, doublecortin, Cell Movement, Animals, Cells, Cultured, Cell Nucleus, Centrosome, Neurons, nucleus, Neuropeptides, Brain, Dyneins, Gene Expression Regulation, Developmental, Cell Differentiation, centrosome, 1-Alkyl-2-acetylglycerophosphocholine Esterase, Mutation, Microtubule-Associated Proteins
Doublecortin Domain Proteins, Doublecortin Protein, Macromolecular Substances, migration, Nervous System Malformations, Microtubules, Article, Lis 1, Mice, doublecortin, Cell Movement, Animals, Cells, Cultured, Cell Nucleus, Centrosome, Neurons, nucleus, Neuropeptides, Brain, Dyneins, Gene Expression Regulation, Developmental, Cell Differentiation, centrosome, 1-Alkyl-2-acetylglycerophosphocholine Esterase, Mutation, Microtubule-Associated Proteins
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