IL‐1β‐dependent activation of NF‐κB mediates PGE2 release via the expression of cyclooxygenase‐2 and microsomal prostaglandin E synthase
Copyright policy )IL‐1β‐dependent activation of NF‐κB mediates PGE2 release via the expression of cyclooxygenase‐2 and microsomal prostaglandin E synthase
Prostaglandin (PG) E2 release is induced in pulmonary A549 cells by the NF‐κB‐activating stimuli interleukin‐1β (IL‐1β) and phorbol 12‐myristate 13‐acetate (PMA). Adenoviral over‐expression of IκBαΔN, a dominant NF‐κB inhibitor, prevents NF‐κB‐dependent transcription and was used to qualify the role of NF‐κB in the release of PGE2. IκBαΔN repressed IL‐1β‐induced, but not PMA‐induced, cycloxygenase‐2 (COX‐2) and microsomal prostaglandin E synthase (mPGES) expression. These data conclusively demonstrate a substantial role for NF‐κB in the co‐ordinate induction of COX‐2, mPGES and in the corresponding release of PGE2 by IL‐1β. However, other pathways are primarily responsible for PGE2 release induced by PMA.
- University of Southampton United Kingdom
- University of Lincoln United Kingdom
- Humboldt-Universität zu Berlin Germany
- University of Warwick United Kingdom
- National Institute of Health Pakistan
570, Transcription, Genetic, Genetic Vectors, Green Fluorescent Proteins, 610, Prostaglandin GH synthase, Dinoprostone, Gene Expression Regulation, Enzymologic, Adenoviridae, Cell Line, Prostaglandin E synthetase, Genes, Reporter, Microsomes, Humans, Nuclear factor κB, Cyclooxygenase-2, Prostaglandin-E Synthases, NF-kappa B, Membrane Proteins, Intramolecular Oxidoreductases, Isoenzymes, Luminescent Proteins, Cyclooxygenase 2, Prostaglandin-Endoperoxide Synthases, Prostaglandins, Tetradecanoylphorbol Acetate, Nuclear factor ?B, Interleukin-1
570, Transcription, Genetic, Genetic Vectors, Green Fluorescent Proteins, 610, Prostaglandin GH synthase, Dinoprostone, Gene Expression Regulation, Enzymologic, Adenoviridae, Cell Line, Prostaglandin E synthetase, Genes, Reporter, Microsomes, Humans, Nuclear factor κB, Cyclooxygenase-2, Prostaglandin-E Synthases, NF-kappa B, Membrane Proteins, Intramolecular Oxidoreductases, Isoenzymes, Luminescent Proteins, Cyclooxygenase 2, Prostaglandin-Endoperoxide Synthases, Prostaglandins, Tetradecanoylphorbol Acetate, Nuclear factor ?B, Interleukin-1
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